OTHER FUNCTIONS - RECOMBINANT ANTIBODIES AS AFFINITY CAPTURES

将基本蛋白质组学发现转化为潜在临床应用的最大障碍之一是缺乏能够从不同类型的基质中可重复地捕获给定蛋白质的稳健且灵敏的试剂。虽然抗体一直是蛋白质捕获的主要参与者，但迫切需要替代试剂来克服与许多当前抗体相关的灵敏度、特异性和再现性的缺乏，以帮助将心血管蛋白质生物标志物的发现转化为临床实用性。开发灵敏的、良好表征的、可重复的和成本有效的试剂，可以捕获涉及心血管生理学或病理学的蛋白质，将极大地有益于心血管研究界。该合同的目标是使用专有的酵母展示抗体库系统产生针对一组心血管标志物的高亲和力抗体。

One of the biggest roadblocks to the translation of basic proteomic discoveries to potential clinical applications is the lack of robust and sensitive reagents that can capture a given protein reproducibly from different types of matrixes. While antibodies have been the major players of protein capture, alternate reagents are urgently needed to overcome the lack of sensitivity, specificity, and reproducibility associated with many of the current antibodies to help translate cardiovascular protein biomarker discoveries into clinical utility. Development of sensitive, well characterized, reproducible and cost effective reagents that can capture proteins involved in cardiovascular physiology or pathology would greatly benefit the cardiovascular research community. The goal of this contract is to generate high affinity antibodies against a panel of cardiovascular markers using a proprietary yeast display antibody library system.