Acquisition of a 500 MHz NMR System for Metabolic Studies

描述（由申请人提供）：通过NIH共享仪器拨款项目，申请用新的安捷伦500 MHz磁铁和控制台替换25年旧的布鲁克500 MHz磁铁和控制台。目前的高分辨率液态核磁共振系统位于耶鲁磁共振研究中心的地下室，该研究中心是耶鲁医学院安利安中心的主要研究大楼的一部分。频繁和重复的硬件故障，以及旧硬件的稳定性和同质性问题，使得在不久的将来必须更换，以保证大量nih资助的赠款的持续生产力。目前的500兆赫系统是与体内磁共振项目密切相关的广泛生化研究的主力。这些包括但不限于血液底物水平和13C分数富集的测量，新型同位素标记底物的开发以及啮齿动物脑提取物样品中代谢物转换的检测。虽然许多高分辨率应用程序可以标记为常规，但它们在为人类和啮齿动物的许多最先进的体内代谢研究提供支持信息方面至关重要。除了新的500 MHz磁铁和控制台外，还需要额外的资金用于具有XYZ梯度的自动调谐RF探针和自动样品转换器。这种组合对于实现最高的生产力和系统利用率至关重要，因为它允许在夜间和周末时间段自动加载、调优、调光和测量数百个类似的样本。目前的500 MHz核磁共振系统支持12个活跃的NIH拨款，在不久的将来有3个未决拨款可能使用该系统。用新的500 MHz系统成功替换当前的500 MHz高分辨率核磁共振系统将为这些NIH拨款提供必要的支持，从而保证持续的生产力和创新。

DESCRIPTION (provided by applicant): Through the NIH Shared Instrument Grant program funds are requested for a replacement of a 25-year old Bruker 500 MHz magnet and console with a new Agilent 500 MHz magnet and console. The current high- resolution, liquid-state NMR system is located in the basement of the Yale Magnetic Resonance Research Center, which is part of the premiere research building, The Anlyan Center, of the Yale School of Medicine. Frequent and repeated hardware failings, as well as stability and homogeneity issues of the old hardware makes a near future replacement mandatory in order to guarantee continued productivity on a large number of NIH-funded grants. The current 500 MHz system is the workhorse for a wide range of biochemical studies closely related to in vivo MR projects. These include, but are not limited to, the measurement of blood substrate levels and 13C fractional enrichments, the development of novel isotopically-labeled substrates and the detection of metabolite turnover in rodent brain extract samples. While many of the high-resolution applications can be labeled as routine, they are of critical importance in providing supporting information for many state-of-the-art in vivo metabolic studies, both in humans and rodents. Besides a new 500 MHz magnet and console, additional funds are requested for an auto-tunable RF probe with XYZ gradients and an automated sample changer. This combination is essential to achieve the highest productivity and system utilization by allowing hundreds of similar samples to be loaded, tuned, shimmed and measured automatically, during both night and weekend time slots. The current 500 MHz NMR system supports twelve active NIH grants, with three pending grants potentially using the system in the near future. A successful replacement of the current 500 MHz high-resolution NMR system with a new 500 MHz system will provide essential support to these NIH grants and will thereby guarantee continued productivity and innovation.