Leica TCS SP5 Multiphoton Confocal Microscope

描述(由申请人提供)：在本申请中，我们建议购买一台激光扫描共聚焦显微镜和多光子成像仪，即徕卡TCS SP5多光子成像仪。该成像仪的目的是1)取代老化的传统激光扫描共聚焦显微镜，2)扩展和升级密西西比大学医学中心(UMMC)的共焦核心成像设备的成像能力。密西西比大学医学中心是密西西比州唯一的学术健康中心，目前密西西比州没有多光子显微镜。UMMC已经组建了一个由NIH资助的研究人员组成的富有成效的小组，他们的研究将随着使用最先进的成像设备而大大推进。目前的共焦核心设施是UMMC在十年前通过购买徕卡TCS SP2而建立的。日常运作，包括例行维护和维修，得到了机构资源和生理学和生物物理系计划项目补助金的支持(PO1 HL051971-18)。共焦核心成像设备一直是我们机构中许多由NIH资助的研究人员的重要研究工具。该设施也可供密歇根大学和当地机构的其他研究人员使用，从而增加了其他研究人员获得竞争性研究资金的机会。共聚焦核心对我们机构的科学研究的发展至关重要；共聚焦成像已被用于阐明结构-功能关系、蛋白质-蛋白质相互作用、离子通量、各种蛋白质的细胞内运输以及量化氧化应激和基因表达的变化。然而，共焦成像技术在过去九年中有了显著的进步，灵敏度、速度和激光穿透率都有所提高。旧仪器的灵敏度限制使我们无法利用高灵敏度、高分辨率的成像功能。此外，我们的老化仪器阻止我们的研究界成员利用新的成像技术，特别是减少组织损伤和光漂白并提供更深入组织成像的多光子成像技术。我们要求的新成像系统是专门为高速、高结构分辨率的共焦成像和活体多光子显微镜而设计的。该仪器将用于整个校园的一系列研究项目，每个项目都针对重大疾病或与健康相关的生理过程。建议的仪器将用于高血压、糖尿病、肾血管生成、神经退行性疾病、肾脏损伤和肾小管功能、红斑狼疮、心脏血管生成、组织修复和癌症的研究。 公共卫生相关性：密西西比大学医学中心的共焦核心成像设备位于医学院生理和生物物理系，由申请人Heather Drummond博士管理，其中包含一台老化的徕卡TCS SP2激光扫描共焦显微镜，购买于2001年。包括生理学和生物物理学、生物化学、药理学、微生物学、解剖学、肾脏病、儿科和牙科学校的成员在内的几个系的成员使用该设施。本申请中要求的新型徕卡SP5多光子成像仪将被集成到密西西比大学医学中心目前的共焦核心成像设备中，并将用于扩展用途并将成像功能(速度、灵敏度、红外)升级为活细胞和整个动物的成像功能。

DESCRIPTION (provided by applicant): In this application, we propose the purchase of a laser scanning confocal microscope and multiphoton imager, the Leica TCS SP5 Multiphoton Imager. The purpose of this imager is to 1) replace an aging conventional laser scanning confocal microscope, and 2) expand and upgrade the imaging capabilities of the Confocal Core Imaging Facility at the University of Mississippi Medical Center (UMMC). The University of Mississippi Medical Center is the only academic health center in Mississippi and there are currently no multiphoton microscopes in the State of Mississippi. UMMC has assembled a productive group of NIH- funded researchers whose research would be greatly advanced with access to state-of-the-art imaging facilities. The current Confocal Core Facility was established ten years ago by UMMC with the purchase of the Leica TCS SP2. Day-to-day operations, including routine maintenance and repairs, have been supported by institutional resources and by the Program Project Grant in the Department of Physiology and Biophysics (PO1 HL051971-18). The Confocal Core Imaging Facility has been an essential research tool to numerous NIH funded investigators at our institution. The facility is also available to other investigators at UMMC and local institutions, thus increasing opportunities for competitive research funding for other investigators. The Confocal Core has been vital to the advancement of scientific research at our institution; confocal imaging has been used to elucidate structure-function relationships, protein- protein interactions, ion flux, intracellular trafficking of various proteins and to quantfy changes in oxidative stress and gene expression. However, confocal imaging technology has advanced significantly in the last nine years with increases in sensitivity, speed and laser penetration. Sensitivity limitations in our older instrument prevent us from taking advantage of high-sensitivity, high-resolution imaging capabilities. Furthermore, our aging instrument prevents members of our research community from taking advantage of new imaging technologies, specifically multiphoton imaging technology which reduces tissue damage and photobleaching and offers imaging deeper into tissues. The new imaging system we are requesting is specifically designed for high-speed, high-structural resolution confocal imaging and intravital multiphoton microscopy. The instrument will be used for a collection of research projects throughout the campus, each directed to significant disease or health-related physiological processes. The proposed instrument will be used in studies addressing hypertension, diabetes, renal angiogenesis, neurodegenerative diseases, renal injury and tubular function, lupus erythematosus, cardiac angiogenesis, tissue repair and cancer. PUBLIC HEALTH RELEVANCE: The Confocal Core Imaging Facility at the University of Mississippi Medical Center is housed in the Department of Physiology and Biophysics in the School of Medicine and managed by the applicant Dr. Heather Drummond and contains an aging Leica TCS SP2 laser scanning confocal microscope purchased in 2001. Members of several departments including Physiology and Biophysics, Biochemistry, Pharmacology, Microbiology, Anatomy, Nephrology, Pediatrics and members of the Dental School use the facility. The new Leica SP5 Multiphoton Imager requested in this application will be integrated into the current Confocal Core Imaging Facility at the University of Mississippi Medical Center and will be used to expand usage and upgrade imaging capabilities (speed, sensitivity, infrared) to those of live cell and whole animal imaging.