PATIENT SAMPLE REPOSITORY AND SPECIALIZED FLOW CYTOMETRY

该共享资源支持两种功能：患者样本库和专门的流式细胞术。核心的存储库功能包括获取适当的患者样本，分离和低温保存单个核细胞，分离和储存血浆或血清，维护注释患者样本的数据库，并将样本分发给项目调查员。我们将通过确保所有样本去识别并分配编码的唯一识别号码来保护患者隐私。我们还将验证是否符合我们的内部监管政策，这些政策规定只能从签署了IRB批准的知情同意文件的患者那里获取和研究样本。核心的流式细胞术功能包括异质样品的多参数分析和高速细胞分选，以获得定义祖细胞群体的高度纯化群体。

This shared resource supports 2 functions: patient sample repository and specialized flow cytometry. The repository functions of the core include the acquisition of appropriate patient samples, isolation and cryopreservation of mononuclear cells, separation and storage of plasma or serum, maintenance of a database annotating patient samples and distribution of samples to program investigators. We will safeguard patient privacy by ensuring that all samples are de-identified and are assigned coded unique identifying numbers. We will also verify compliance with our internal regulatory policies which stipulate that samples may be obtained and studied only from patients who have signed an IRB approved informed consent document. The flow cytometry functions of the core include multiparameter analysis of heterogeneous samples and high-speed cell sorting to obtain highly purified populations of defined progenitor populations. The specific aims of this core are listed below: 1. To ensure the maintenance of patient privacy and to verify that informed consent has been obtained for acquisition and study of all samples. 2. To ensure the acquisition of appropriate samples as designated in clinical research protocols carried out by program investigators. 3. To isolate and cryopreserve mononuclear cells and plasma or serum components from patient samples for subsequent study by program investigators. 4. To maintain an annotated database containing pathologic, cytogenetic and molecular information for clinical samples obtained from patients with myeloid leukemias. 5. To ensure that samples are distributed in a timely and equitable fashion to laboratory investigators for experiments proposed in this application. 6. To provide specialized multi-parameter analysis and high-speed cell sorting to obtain highly purified populations for functional and genetic analysis