CORE SERVICE FOR IMMUNOLOGIC ASSAYS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该中心应要求为内部和外部研究人员的特定研究项目提供免疫学服务。目前的服务包括在最佳检测分析的标准化程序下的样品制备、ELISPOT检测的规划和执行、数据处理和演示、细胞内细胞因子染色，以及在这些检测中用作抗原刺激剂的重组牛痘病毒库存的制备。我们继续使用Mabtech Elispot系统以及U-Cytech系统，具体取决于特定的项目和合作者。在很大程度上，我们继续使用Zellnet咨询服务来记录和分析Elispot的结果。细胞免疫学核心还为研究人员提供MHC分型服务。已经实施了自动收费系统。为了继续扩展我们的服务，我们现在为MHC分型列表提供了总共10个等位基因。在这一年里，TNPRC分配给不同项目的恒河猴接受了MHC-I和II基因的检测。核心继续向调查人员提供恒河猴MHC I类四分仪。分析总数包括：A*01=1 952；A*02=1 907；A*08=1 869；A*11=1 869；B*01=1 817；B*03=1 875；B*04=1 870；B*08=1 929；B*17=1 930；DRB*w201=1 833；总计18885次。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Core provides immunology services to specific research projects of in-house and outside investigators as requested. Current services include sample preparation under standardized procedures for optimal assay analysis, planning and performance of ELISPOT assays, data processing and presentation, intracellular cytokine staining, and preparation of recombinant vaccinia viral stocks for use as antigenic stimulants in these assays. We continue to use the MabTech ELISPOT system as well as the U-Cytech system depending on the particular project and collaborators. For the most part we have continued to use the Zellnet Consulting service for recording and analyzing the ELISPOT results. The Cellular Immunology Core also provides MHC typing service to investigators. An automated charging system has been implemented. Continuing to expand our services, we now offer a total of 10 alleles to the MHC typing list. During this year, Rhesus monkeys assigned to different projects at the TNPRC were tested for the presence of Rhesus monkey MHC-I and II genes. The core continues to provide Rhesus MHC Class I tetrameters to investigators. Total numbers of assays include: A*01 = 1952; A*02 = 1907; A*08 = 1869; A*11 = 1869; B*01 = 1817; B*03 = 1875; B*04 = 1870; B*08 = 1929; B*17 = 1930; DRB*w201 = 1833; for a grand total of 18885 assays.