PRIMATE MODEL FOR MYCOPLASMA GENITALIUM

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在我们的研究基金IR21AI074898 "生殖支原体的灵长类动物模型"中，我们评估了猪尾猕猴作为研究生殖支原体免疫发病机制的动物模型的能力。生殖器炎（MG）是一种新发现的生殖道病原体。 在该模型中，用MG对灵长类动物进行宫颈接种，然后以跨越8周的间隔收集阴道和宫颈样本，用于通过qPCR和培养检测MG。 在第4周（输卵管和伞部）和第8周（输卵管、伞部和子宫颈）收集活组织检查，此时对动物进行治疗以治愈MG感染。 对活检组织进行培养、qPCR和组织学处理，以检测免疫细胞浸润。 在感染前每隔一段时间收集血清，以评估感染过程中抗MG抗体的产生。 在过去的一年中，我们已经用MG接种了该提案资助的6只灵长类动物中的4只：在整个8周的研究中，2只灵长类动物成功定植，1只定植4 - 6周，第4只动物未感染。 血清的初步分析表明，抗体MG感染后产生的特异性识别的免疫显性粘附分子，MgpB和MgpC。上生殖道组织的培养和qPCR表明，MG尚未从这些动物的下生殖道上升，这表明可能需要更长时间的实验或重复接种以诱导上生殖道感染。最后，正在进行实验以评估在感染期间响应于抗体产生的MgpB和MgpC中的序列变异。 最后两个由这笔赠款资助的灵长类动物计划在2011年2月至8月接受我们的方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In our grant, IR21AI074898, "A Primate Model of Mycoplasma genitalium" we are assessing the ability of pigtail macaques to serve as an animal model to study the immunopathogenesis of M. genitalium (MG), a newly recognized reproductive tract pathogen. In this model primates are inoculated cervically with MG then vaginal and cervical specimens are collected at intervals spanning 8 weeks for detection of MG by qPCR and culture. Biopsies are collected at Week 4 (Fallopian tube and fimbriae) and Week 8 (Fallopian tube, fimbriae and cervix) at which time the animals are treated to cure the MG infection. The biopsies are processed for culture, qPCR and histology to detect immune cell infiltration. Serum is collected prior to infection at and intervals to assess the production of anti-MG antibodies over the course of the infection. In the past year we have inoculated 4 of the 6 primates funded by this proposal with MG: 2 primates were successfully colonized throughout the 8 weeks of the study, one was colonized for 4-6 weeks and the fourth animal was not infected. Preliminary analysis of serum suggests that antibodies to MG are produced after infection that specifically recognized the immunodominant adhesin molecules, MgpB and MgpC. Culture and qPCR of the upper reproductive tract tissues suggests that MG has not ascended from the lower tract in these animals suggesting that longer experiments or repeated inoculations may be necessary to induce upper tract infection. Finally, experiments are underway to assess sequence variation in MgpB and MgpC during infection in response to antibody production. The final two primates funded by this grant are scheduled to undergo our protocol, scheduled for Feb-Aug 2011.