MESENCHYME?EMBRYONIC STEM CELL INTERACTIONS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 目的：探讨基质细胞对体外培养滋养层细胞分化的影响。 人类胚胎干细胞。 进度： 由于局限性，人类早期胎盘发育的研究是困难的 关于围植入期实验材料的可用性。我们利用了一个 三维共培养系统测定不同效应细胞的效应 滋养层细胞分化的类型。表达绿色荧光蛋白的H1人胚胎干细胞 (H1EGFP HESC)与红细胞追踪器标记的人 足月胎盘成纤维细胞(TPF)、绒毛样本成纤维细胞(CVS)或真皮 成纤维细胞(CI2F)形成组合类胚体(EBS)，目标是 胎盘并入概述胎盘形态发生 间充质细胞。这些EBS在悬浮培养中保持30天。 每天收集培养液进行激素测定，并固定和处理EBS 免疫组织化学染色检测细胞角蛋白和hCG- 不同处理组的阳性细胞数。总体而言，结果表明 利用间充质细胞从足月胎盘(TPF)制备EB时， EB来源的联合滋养层细胞分泌更高水平的hCG和孕酮 与没有效应器细胞的EBS相比。这是由于……的分泌活动 EB来源的滋养层细胞与未分化的滋养层细胞数量无关 每EB的滋养层细胞，表明胎盘的非滋养层细胞可以 影响滋养层内分泌活动。 这项工作使用了WNPRC干细胞资源。 出版物： 首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容 2010胎盘来源的间充质细胞对绒毛膜促性腺激素和 人胚胎干细胞来源的滋养层细胞的孕酮分泌。 生殖科学。17(9)：798-808。20601539。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Objective: To define the influence of stromal cells on trophoblast differentiation from human embryonic stem cells. PROGRESS: Studies of early placental development in humans are difficult because of limitations on experimental material availability from the perimplantation period. We utilized a three-dimensional co-culture system to determine the effects of various effector cell types on trophoblast differentiation. EGFP-expressing H1 human embryonic stem cells (H1EGFP hESC) were used in co-suspension with red Cell Tracker-labeled human term placental fibroblasts (TPF), chorionic villus sampling fibroblasts (CVS), or dermal fibroblasts (CI2F) to form combination embryoid bodies (EBs), with the goal of recapitulating placental morphogenesis through incorporation of placental mesenchymal cells. These EBs were maintained in suspension culture for 30 days. Media were collected daily for hormone assay, and EBs were fixed and processed for immunohistochemical staining to determine the number of cytokeratin and hCG- positive cells in the different treatment groups. Overall, the results demonstrated that when utilizing mesenchymal cells for EB preparation from term placentas (TPF), combination EB-derived trophoblasts secrete higher levels of hCG and progesterone compared to EBs made without effector cells. This is due to the secretory activity of the EB-derived trophoblasts and not due to the number of differentiated trophoblasts per EB, demonstrating that nontrophoblast cells of the placenta can influence trophoblast endocrine activity. This work used WNPRC Stem Cell Resources. PUBLICATIONS: Giakoumopoulos, M., Siegfried, L.M. Dambaeva, S.V., Garthwaite, M.A., and T.G. Golos. 2010 Placental-derived mesenchyme influences chorionic gonadotropin and progesterone secretion of human embryonic stem cell-derived trophoblasts. Reproductive Sciences. 17(9):798-808. PMID: 20601539.