PROOF OF CONCEPT FOR CARD-BASED CD4 CELL COUNTING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 第二阶段SBIR应用将继续开发用于监测CD 4+的廉价护理点卡/阅读器系统 艾滋病毒感染者的细胞，用于资源贫乏地区。 该技术使用薄膜层压微流体盒来收集， 在储存所需检测试剂的同时，对血样进行计数和储存，并从台式单元注入试剂卡。 其目的是系统地开发和故障排除设备，最终验证其读数与标准相比 流式细胞术使用来自人类受试者的全血计数小体积的CD 4+细胞，每次测试不到1.50美元。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This phase II SBIR application will continue the development of an inexpensive point-of-care card/reader system for monitoring CD4+ cells in HIV-infected subjects, for use in resource poor areas. The technology uses a thin-film laminated microfluidic cassette to collect, count and store the blood sample while the required detecting reagents are stored and injected into the cassettes from the bench top unit. The aims are designed to systematically develop and troubleshoot the device, ultimately validating its readings comparing with standard flow cytometry using whole blood from human subjects to enumerate CD4+ cells in small volumes for less than $1.50 per test.