Wisconsin National Primate Research Center Support

描述(由申请人提供)：了解控制艾滋病病毒复制的免疫学和遗传学基础应有助于指导疫苗设计。与感染艾滋病毒的人类一样，有限数量的猕猴在感染后自发地将SIV复制控制在1000拷贝/毫升以下(“精英控制者”；ECs)(18，32)。这比使用SIVmac239攻击后的典型血浆病毒载量减少了两个对数以上。最近，我们在196只印度恒河猴队列中发现了16只ECs(18只)。MHC-I类等位基因分型显示，16例ECs中有14例表达MAMU-B*17或MAMU-B*08，它们分别与HLAB57和HLAB27在功能上相当(2、9、12、21、26、29)。然而，对这些动物的监测和采样通常是在不同的调查人员进行的实验过程中确定的，但并不一致。此外，由于资金紧张，动物往往必须在具体研究结束时实施安乐死。我们计划建立一个样本库，数据库，并在WNPRC容纳现有和未来的EC猕猴。将这些独特的资源提供给从事小岛屿发展中国家工作的研究人员群体，将是对该领域的一项极其宝贵的服务。

DESCRIPTION (provided by applicant): Understanding the immunological and genetic basis of control of AIDS virus replication should help inform vaccine design. As in HIV-infected humans, a limited number of macaques spontaneously control SIV replication to less than 1,000 copies/ml after infection ("Elite Controllers"; ECs) (18, 32). This is a greater than two log reduction from the typical plasma virus load after challenge with SIVmac239. Recently we identified sixteen ECs in a cohort of 196 Indian rhesus macaques (18). Genotyping for MHC class I alleles revealed that fourteen of these sixteen ECs expressed either Mamu-B*17 or Mamu-B*08, which appear to be the macaque functional equivalents of HLA-B57 and HLA-B27, respectively (2, 9, 12, 21, 26, 29). However, the monitoring and sampling of these animals, which were typically identified in the course of experiments run by different investigators, has not been consistent. Moreover, due to financial constraints, animals often must be euthanized at the end of specific studies. We plan to establish a sample bank, database, and to house existing and future EC macaques at the WNPRC. Making these unique resources available to the community of investigators working on SIV would be an extremely valuable service to the field.