FORE-SCI TRAINING PROGRAM (RFP 08-01)

本建议的主要目的是提供一个脊髓损伤（SCI）研究技术的年度培训计划。参加该计划的人员将在以下方面接受深入的实验室实践培训：胸部和颈部椎板切除术，大鼠和小鼠的脊柱挫伤和横断损伤，脊柱挫伤器械操作，地上运动分析（BBB和BMS）、先进的行为技术、SCI大鼠和小鼠的护理、脊髓组织的组织学制备和显微镜分析、电生理读数本发明涉及用于药物和/或细胞的脊柱内递送的方法（包括EMG、MEP和SEP）、用于药物和/或细胞的脊柱内递送的方法，包括显微注射和鞘内导管递送。该计划还将强调SCI的人性化方面，包括SCI委员会认证的神经学家的讲座和与SCI患者的互动讨论，讨论他们在日常生活中面临的问题。我们还将通过讲座和讨论等主题提供相关信息，如SCI研究的历史背景，实验设计，数据分析和解释，tract tracing以及其他主题。所有学员将获得广泛的课程材料，包括一个插图课程手册和几个DVD包含运动分析相关信息。该计划的目标是，所有参与者在完成计划后将精通SCI研究，并能够将OSU培训计划中学到的技术传授给他们家庭实验室的其他成员。

The major objective of this proposal is to provide an anual training program in spinal cord injury (SCI) research techniques. Participants in this program will receive in depth hands-on laboratory training in: Thoracic and cervical laminectomy surgeries, spinal contusion and transection injuries in rats and mice, operating spinal contusion injury devices, overground locomotor analysis (BBB and BMS), advanced behavioral techniques, care of SCI rats and mice, histological preparation and microscopic analysis of spinal tissue, electrophysiological readouts (including EMGs, MEPs, and SEPs), methods for intraspinal delivery of drugs and/or cells including microinjections and intrathecal catheter delivery. This program will also emphasize the human aspect of SCI by including a lecture from a SCI board certified neurologist and several interactive discussions with individuals with SCI in which the issues they face in daily life will be discussed. We also will provide relevant information via lectures and discussions on such topics as the historical background on SCI research, experimental design, data analysis and interpretation, tract tracing as well as other topics. All trainees will receive extensive course materials including an illustrated course manual and several DVDs containing locomotor analysis related information. It is the goal of the program that all participants will be proficient in performing SCI research after completion of the program and will be able to teach the techniques learned in the OSU training program to other members of their home laboratory.