COBRE: LSU: IMAGING CORE RESOURCE MODULE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们设施中的双光子激光扫描显微镜配备了两个振镜扫描器，每个扫描器独立地引导来自超快激光的两束光束。这两种扫描仪都在定制软件的控制下，允许同时释放各种笼状化合物和相关信号的双光子成像以及电子记录。优异的空间分辨率、组织穿透性和低光毒性的组合使该显微镜可以在广泛的实验中使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The two-photon laser-scanning microscope available in our facility is equipped with dual galvonometeric laser scanners with each of these scanners independently directing two beams from ultrafast lasers. Both scanners are under the control of custom software allowing for simultaneous photo-release of various caged compounds and two-photon imaging of associated signals as well as electrical recordings. The combination of excellent spatial resolution, tissue penetration and low phototoxicity allows the microscope to be used in a wide range of experiments.