TRANSGENIC INHIBITION OF MICRO-RNAS IN MOUSE BRAIN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 高分辨率磁共振显微镜将有助于评估这种新品系小鼠可能的形态变化。 我们的假设是，这些新产生的小鼠是亨廷顿氏病的模型，我们将使用磁共振通过评估与亨廷顿氏病有关的已知区域的形态变化来检验这一假设，最突出的是纹状体。现有的研究很少评估亨廷顿病小鼠模型的形态计量学变化，这些研究包括很少的大脑大区域(Mori，2010，Fox 2009)。此外，自动分割将提供包括海马体、下丘脑和新皮质在内的其他区域在该小鼠模型中是否萎缩的信息。因此，我们期望高分辨率MRM的使用将允许对这一新品系的小鼠进行全面的解剖表型分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. High resolution MR microscopy will help assess possible morphological alterations in this new strain of mice. Our hypothesis is that these newly generated mouse is a model for Huntington's disease and we are going to use MR to test this hypothesis by assessing morphometric changes in regions known to be involved in Huntington's disease, most prominently the the striatum. Few exisitng studies have assessed morphometric changes in mouse model's of Huntington's disease and these studies include few large regions of the brain (Mori, 2010, Fox 2009). In addition, automated segmentation will offer information on whether other regions, including the hippocampus, hypothalamus, and neocortex are atrophied in this mouse model. We expect thus that the use of high resolution MRM will allow a comprehensive anatomical phenotyping of this new line of mice.