GD STAINING/MRI FOR FLIGHT MUSCLES ANATOMY IN FORMALIN-FIXED LARGE BEETLE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 未解剖的大型雌性甲虫（来自亚利桑那州图森市的帕洛韦尔迪根螟（Derobrachus sp））的前2/3的Gd染色和MRI图像，先前用（且仍在）10%福尔马林林格氏溶液固定。这将补充2009年7月的MRI研究和后来对同一物种的未固定、未染色的雄性和雌性甲虫的解剖。 目的有两个：1）对胸部的纵肌和背腹飞行肌的位置和纤维大小和方向进行成像， 2)验证在狭窄的头部和宽敞的胸腔和腹腔中明显和明显缺乏a）功能性口腔部分，B）吞咽通道（吞咽通道）和任何胃肠道体征，同时未受任何先前侵入性解剖的干扰。 想讨论高分辨率MRI成像的固定飞行肌肉原位在胸部从一个巨大的水蝽，也许与Gd染色，以补充我的2000年的研究未固定的飞行肌肉原位在整个水蝽。 这些肌肉通过X射线微束扫描显示出长程结晶，但通过EM薄切片不能显示;我们想做MRI，然后塑料包埋并对整个飞行肌肉的未扰动原位结构进行光学显微镜检查，以观察长程结晶和暗示的肌原纤维的全部或任何扭曲的缺失是否可以通过薄切片EM被仔细保存和可视化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Gd stain and MRI image of anterior 2/3 of undissected large female beetle (Palo Verde Root Borer (Derobrachus sp) from Tucson, AZ), previously fixed by (and still in) 10% formalin in Ringer solution. This will complement July 2009 MRI study and later dissections of unfixed, unstained male & female beetles of same species. Objectives are twofold: 1) to image position and fiber size & orientation of lomgitudinal and dorso-ventral flight muscles in thorax, and 2) to verify apparent and surprisig absence of a) functional mouth parts, b) swallowing passage (esophgus) and any sign of GI tract in narrow cephalic and spacious thoracic and abdominal cavities while undisturbed by any prior invasive dissection. Want to discuss high-resolution MRI imaging of aldehyde-fixed flight muscle in situ in thorax from a giant waterbug, perhaps with Gd-staining, to complement my year 2000 study of unfixed flight muscle in situ in a whole waterbug. These muscles show long-range crystallinity by x-ray microbeam diffractio, but not by EM thin sectioning; We want to do MRI and then plastic embed and do light microscopy of unperturbed in-situ structure of whole flight muscle to see if the long-range crystalline and the implied absence of all or any twisitng of myofibrils can be carefully preserved and visualized by thin-section EM.