NIR ENZYME-ACTIVATED FLUOROGENIC PHOSPHOLIPIDS FOR MULTI-MODALITY IMAGING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 磷脂酶催化膜磷脂的水解，进而产生一种 多种脂类第二信使。这些脂质衍生产品对这两种疾病都是必要的 生理和病理过程。理解磷脂代谢，因为它 与癌症有关，对于诊断应用和开发新的靶向是必要的 化疗。传统上，脂质代谢物的变化是通过磁学观察的。 共振光谱(MRS)或通过层析方法。使用以下组合 利用近红外(NIR)荧光分子探针，我们的目标是磷脂酶 功能的目标是描绘体内特定的细胞信号通路。这些信息 从分子成像方法中获得的信息可能会导致早期诊断和改善 疾病的管理。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phospholipases catalyze the hydrolysis of membrane phospholipids, which, in turn, generates a variety of lipid second messengers. These lipid-derived products are necessary for both physiological and pathological processes. An understanding of phospholipid metabolism, as it relates to cancer, is necessary for diagnostic applications and for developing novel targeted chemotherapy. Traditionally, lipid metabolite changes have been observed by magnetic resonance spectroscopy (MRS) or by chromatographic methods. Using a combination of MRS with near-infrared (NIR) fluorescent molecular probes we are targeting phospholipase function with the goal of delineating specific cell signaling pathways in vivo. The information gained from a molecular imaging approach may lead to earlier diagnoses and improved management of disease.