HIGH RESOLUTION IMAGING OF MYO-INSITOL IN ALZHEMIER?S DISEASE PATHOLOGY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 肌醇(MI)是人脑中含量最丰富的代谢产物之一。MI是神经胶质细胞增殖的标志，在脑内起渗透调节作用。MI的浓度已被证明在不同的大脑病理中发生变化。质子磁共振波谱(1HMRS)研究表明，阿尔茨海默病(AD)患者的MI浓度增加。胶质细胞的增殖/激活与AD的病理改变密切相关。1HMRS已被广泛用于监测AD的MI变化，但分辨率较低，也不能提供胶质细胞增殖/激活较高的区域的信息。最近，利用化学交换饱和转移成像(CEST)技术，利用MI上存在的可交换的OH质子，实现了人脑MI的高分辨率映射。在本研究中，我们首次在转基因AD小鼠(APP/PS1)上进行MI CEST，以评估与野生型小鼠相比MI浓度的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Myo-Inositol (MI) is one of the most abundant metabolites present in the human brain. MI is a marker of glial cells proliferation and functions as an osmolyte in brain. The concentration of MI has been shown to change in various brain pathologies. Proton magnetic resonance spectroscopy (1HMRS) studies have shown increased MI concentration in Alzheimer's disease (AD). Glial cells proliferation/activation is associated with the pathological changes in AD. 1HMRS has been widely used to monitor MI changes in AD, however, it suffers from poor resolution and also does not provide information about the region with the higher glial cells proliferation/activation. Recently, high-resolution mapping of MI in human brain has been performed by exploiting exchangeable OH protons present on MI using a technique commonly known as chemical exchange saturation transfer imaging (CEST). In the current study, for the first time we perform MI CEST on transgenic AD mice (APP/PS1) to evaluate the changes in MI concentration compared to wild type mice.