CEST EFFECT FROM PHOSPHO-CREATINE (PCR) AND ADENOSINE-TRI-PHOSPHATE (ATP)

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 磷酸肌酸（PCr）和三磷酸腺苷（ATP）是生物系统中的主要能量来源/储存库。ATP和PCr的浓度在不同的器官中是不同的。脑、心脏和肌肉中的ATP浓度分别为~3- 4 mM、~ 6 mM和~ 10 mM。另一方面，PCr是能量库的主要来源，在大脑中存在约5- 6 mM，在心脏中存在10 mM，在肌肉中存在约33.5mM。这些富含能量的化合物的变化已被报道在各种疾病中，包括肿瘤、心脏梗塞和肌肉疾病。~（31）P MRS已被广泛用于检测这些代谢物的信号。先前已经报道了利用Pi和PCr之间的MT效应的PCr的磷成像。在这里，我们第一次报告的化学交换饱和转移（CEST）的影响，从PCr和ATP在体外的phantomy通过利用其不稳定的胺质子（-NH 2），以及PCr MRI CEST地图从休息小腿肌肉。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phospho-creatine (PCr) and Adenosine tri-phosphate (ATP) are the major energy sources/reservoirs in the biological system. The concentration of ATP and PCr is varied in the different organs. The ATP concentration in brain, heart and muscle is ~3-4mM, ~6mM and ~10mM, respectively. On the other hand PCr which is a major source of energy reservoir present around 5-6mM in brain, 10mM in heart and around 33.5mM in muscles. Alternations of these energy-rich compounds have been reported in various disease conditions including tumors, heart infarction and muscular disorders. 31P MRS has been widely used to detect the signal from these metabolites. Phosphorous imaging of PCr has been previously reported utilizing MT effects between Pi and PCr. Here, for the first time we report the chemical exchange saturation transfer (CEST) effect from PCr and ATP in-vitro on phantoms by exploiting their labile amine protons (-NH2) as well as a PCr MRI CEST map from resting calf muscle.