CEST EFFECT FROM PHOSPHO-CREATINE (PCR) AND ADENOSINE-TRI-PHOSPHATE (ATP)

磷酸肌酸 (PCR) 和三磷酸腺苷 (ATP) 的 CEST 效应

• 批准号: 8362002
• 负责人: Mohammad Haris
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362002
• 关键词: Adenosine; Amines; Brain; Chemicals; Creatine; Disease; Energy-Generating Resources; Funding; Grant; Heart; Image; In Vitro; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Muscle; Myocardial Infarction; Myocardium; Myopathy; National Center for Research Resources; Organ; Phosphorous; Principal Investigator; Protons; Reporting; Research; Research Infrastructure; Resources; Rest; Signal Transduction; Source; Time; United States National Institutes of Health; biological systems; cost; inorganic phosphate; optical imaging; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phospho-creatine (PCr) and Adenosine tri-phosphate (ATP) are the major energy sources/reservoirs in the biological system. The concentration of ATP and PCr is varied in the different organs. The ATP concentration in brain, heart and muscle is ~3-4mM, ~6mM and ~10mM, respectively. On the other hand PCr which is a major source of energy reservoir present around 5-6mM in brain, 10mM in heart and around 33.5mM in muscles. Alternations of these energy-rich compounds have been reported in various disease conditions including tumors, heart infarction and muscular disorders. 31P MRS has been widely used to detect the signal from these metabolites. Phosphorous imaging of PCr has been previously reported utilizing MT effects between Pi and PCr. Here, for the first time we report the chemical exchange saturation transfer (CEST) effect from PCr and ATP in-vitro on phantoms by exploiting their labile amine protons (-NH2) as well as a PCr MRI CEST map from resting calf muscle.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 磷酸肌酸（PCr）和三磷酸腺苷（ATP）是生物系统中的主要能量来源/储存库。 ATP和PCr的浓度在不同器官中是不同的。大脑、心脏和肌肉中的 ATP 浓度分别为~3-4mM、~6mM 和~10mM。另一方面，PCr 是能量储存的主要来源，在大脑中含量约为 5-6mM，在心脏中含量约为 10mM，在肌肉中含量约为 33.5mM。据报道，这些富含能量的化合物在多种疾病中发生变化，包括肿瘤、心脏病和肌肉疾病。 31P MRS 已广泛用于检测这些代谢物的信号。先前已有报道利用 Pi 和 PCr 之间的 MT 效应进行 PCr 磷成像。在这里，我们首次通过利用其不稳定的胺质子 (-NH2) 以及来自静息小腿肌肉的 PCr MRI CEST 图，报告了体外 PCr 和 ATP 对模型的化学交换饱和转移 (CEST) 效应。