CELL WALL ANALYSIS OF BRACHYPODIUM TRANSGENES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 短柄草样品已经与CAD和PAL缺陷转基因系一起生长以改善它们的消化率。将它们球磨并在DMSO-吡啶中制备。将采用1H-13 C HSQC光谱来检查该突变对样品细胞壁的影响。将与Ralph实验室合作进行化学计量分析，以确定样品之间细胞壁的任何相关变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Brachypodium samples have been grown with CAD and PAL deficient transgene lines to improve their digestibility. These were be ball milled and prepared in DMSO-pyridine. 1H-13C HSQC spectra will be taken to examine the effects of this mutation on the cell wall of the samples. Analysis will be done chemometrically in collaboration with the Ralph lab to determine any relevant changes in the cell wall between samples.