HEPARIN STRUCTURE STUDY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 肝素因其抗凝血活性而广为人知，因为它能够加速抗凝血酶抑制血液凝固级联中丝氨酸蛋白酶的速度。肝素是一种线性聚合物，由1？4连接的吡喃糖醛酸和氨基葡萄糖残基的重复单元组成，含有许多给予它高负电荷的磺基。核磁共振是一种强大的技术，能够研究糖胺多糖的结构，而不需要事先通过化学或酶作用将其裂解成较小的寡糖。因此，核磁共振可以用来避免当多糖被分解成较小的寡糖时导致的结构信息的损失。在这里，我们用质子和碳13核磁共振研究了肝素的结构和不同制备批次之间的差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Heparin is well-known for its anticoagulant activity based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin is a linear polymer consisting of repeating units of 1¿4 linked pyranosyluronic acid and glucosamine residues and contains many sulfo groups that give it a high negative charge. NMR is a powerful technique capable of studying the structure of glycosaminoglycans without prior chemical or enzymatic cleavage into smaller oligosaccharides. Thus, NMR can be used to avoid the loss of structural information that results when the polysaccharides are broken down to smaller oligosaccharides. Here we performed proton and carbon 13 NMR to study the structure of heparin and the difference among different preparative lots.