TRYPANOSOMA FLAGELLUM

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 霸王布鲁氏菌鞭毛是一种多组分、多功能的细胞器，对致病至关重要。该鞭毛不仅含有典型的“9+2”轴丝，而且还含有一个结晶的鞭毛旁杆（PFR）。该项目的生物学兴趣是PFR的两个主要结构组分的结构组织，其已被表征，分别为69 KD和73 KD的PFR 1和PFR 2。通过序列分析，它们似乎主要由卷曲螺旋基序组成。通过RNA干扰耗尽一种PFR蛋白并不影响寄生虫的生长或分裂，也不影响轴丝的形成。然而，它完全消除了PFR的组装，导致细胞运动性和感染性的缺陷。我们计划使用冷冻ET来研究这个复杂细胞器的结构组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The T. brucei flagellum is a multi-component, multifunctional organelle critical for pathogenesis. This flagellum contains not only the classic "9+2" axoneme but also a crystalline paraflagellar rod (PFR). The biological interest in this project is the structural organization of the two major structural components of PFR which have been characterized, PFR1 and PFR2 at 69KD and 73KD, respectively. They appear by sequence analysis to consist largely of coiled-coil motifs. Depletion of one PFR protein by RNA interference did not affect parasite growth or division nor formation of the axoneme. However, it completely abolished the assembly of the PFR, resulting in defects in cell motility and infectivity. We plan to use cryo-ET to study the structural organization of this complex organelle.