TRYPANOSOMA FLAGELLUM

鞭毛锥虫

• 批准号: 7953812
• 负责人: Cynthia He
• 金额: $ 3.48万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953812
• 关键词: Affect; Biological; Computer Retrieval of Information on Scientific Projects Database; Defect; Electron Microscopy; Flagella; Funding; Grant; Growth; Institution; Organelles; Parasites; Pathogenesis; Proteins; RNA Interference; Research; Research Personnel; Resources; Sequence Analysis; Source; Trypanosoma; Trypanosoma brucei brucei; United States National Institutes of Health; cell motility; interest; macromolecule; retinal rods

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The T. brucei flagellum is a multi-component, multifunctional organelle critical for pathogenesis. Flagellum in Trypanosoma brucei contains not only the classic "9+2" axoneme but also a crystalline paraflagellar rod (PFR). The biological interest in this project is the structural organization of the two major structural components of PFR which have been characterized, PFR1 and PFR2 at 69KD and 73KD, respectively (Deflorin et al., 1994). They appear by sequence analysis to consist largely of coiled-coil motifs. Depletion of one PFR protein by RNA interference did not affect parasite growth or division nor formation of the axoneme. But it completely abolished the assembly of the PFR, resulting in defects in cell motility and infectivity (Bastin et al., 1998; Bastin et al., 1999).

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 布鲁氏链球菌鞭毛是一种多功能的多功能细胞器，对发病机理至关重要。锥虫瘤中的鞭毛不仅包含经典的“ 9+2”轴突，还包含晶体式翼型杆杆（PFR）。该项目的生物学兴趣是PFR的两个主要结构成分的结构组织，分别在69kD和73kD时表征了PFR1和PFR2（Deflorin等，1994）。它们通过序列分析出现，主要由盘绕螺旋序列组成。 RNA干扰对一种PFR蛋白的耗竭不会影响寄生虫的生长或分裂或轴突形成。但这完全消除了PFR的组装，从而导致细胞运动和感染性缺陷（Bastin等，1998； Bastin等，1999）。