TRYPANOSOMA FLAGELLUM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 布鲁氏鞭毛是一种多组分、多功能的细胞器，在致病过程中起着至关重要的作用。布氏锥虫的鞭毛不仅含有经典的“9+2”轴丝，还含有晶状的旁纤维杆(PFR)。该项目的生物学兴趣是PFR的两个主要结构成分的结构组织，这两个主要结构成分的特征分别是69KD和73KD处的PFR1和PFR2(Deflorin等人，1994年)。通过序列分析，它们似乎主要由卷曲的线圈基序组成。通过RNA干扰耗尽一个PFR蛋白并不影响寄生虫的生长或轴丝的分裂或形成。但它完全取消了PFR的组装，导致细胞运动性和传染性的缺陷(Bastin等人，1998；Bastin等人，1999)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The T. brucei flagellum is a multi-component, multifunctional organelle critical for pathogenesis. Flagellum in Trypanosoma brucei contains not only the classic "9+2" axoneme but also a crystalline paraflagellar rod (PFR). The biological interest in this project is the structural organization of the two major structural components of PFR which have been characterized, PFR1 and PFR2 at 69KD and 73KD, respectively (Deflorin et al., 1994). They appear by sequence analysis to consist largely of coiled-coil motifs. Depletion of one PFR protein by RNA interference did not affect parasite growth or division nor formation of the axoneme. But it completely abolished the assembly of the PFR, resulting in defects in cell motility and infectivity (Bastin et al., 1998; Bastin et al., 1999).