THE EFFECTS OF PHYTOSTEROLS PRESENT IN NATURAL FOOD MATRICES ON CHOLESTEROL

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 背景/目的：饮食中添加外源性植物类固醇可降低肠道胆固醇吸收和血浆低密度脂蛋白-胆固醇。然而，当它们以天然的、未经纯化的形式和在饮食中可达到的量给药时，对胆固醇代谢的影响知之甚少。这项研究的目的是检验未经修饰的食物中存在的内在植物类固醇改变全身胆固醇代谢的假设。对象/方法：总共24名受试者中的20人完成了随机交叉喂养试验，其中所有的食物都由代谢厨房提供。每个受试者在4周的时间里吃两次饮食。两种饲料的植物甾醇含量不同(缺乏植物甾醇的饲料，植物甾醇126毫克/2000千卡；富含植物甾醇的饲料，植物甾醇449毫克/2000千卡)，但在其他方面营养含量是匹配的。口服稳定同位素示踪剂后，用气相色谱/质谱仪测定胆固醇的吸收和排泄。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background/Objectives:Extrinsic phytosterols supplemented to the diet reduce intestinal cholesterol absorption and plasma low-density lipoprotein (LDL)-cholesterol. However, little is known about their effects on cholesterol metabolism when given in native, unpurified form and in amounts achievable in the diet. The objective of this investigation was to test the hypothesis that intrinsic phytosterols present in unmodified foods alter whole-body cholesterol metabolism.Subjects/Methods:In all, 20 out of 24 subjects completed a randomized, crossover feeding trial wherein all meals were provided by a metabolic kitchen. Each subject consumed two diets for 4 weeks each. The diets differed in phytosterol content (phytosterol-poor diet, 126 mg phytosterols/2000 kcal; phytosterol-abundant diet, 449 mg phytosterols/2000 kcal), but were otherwise matched for nutrient content. Cholesterol absorption and excretion were determined by gas chromatography/mass spectrometry after oral administration of stable isotopic tracers.