DIETARY CONTENT & GASTROINTESTINAL ABSORPTION OF D CHIRO INOSITOL & PINITOL

膳食含量

• 批准号: 6665877
• 负责人: RICHARD E. OSTLUND
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665877
• 关键词: biological products; biomedical resource; human tissue; immunology; inflammation; proteins; spectrometry

D-chiro-Inositol-containing compounds were abundant in common laboratory animal feed. By gas chromatography-mass spectrometry (GC/MS) of 6 N HCI hydrolysates, Purina Roden Chow 5001 contained 0.23% of D-chiro-inositol by weight. The principal sources were alfalfa and soybean meal whereas other chow ingredients had less than 2% as much per gram as these legumes. Only traces of L-chiro-inositol were found. D-chiro-Inositol was present in a water-soluble non-ionic form of which most was shown to be pinitol, 1D-3-O-methyl-chiro-inositol. More than 98% of the D-chiro-Inositol fed to rats as Purina Rodent Chow 5001 as well as purified pinitol and deuterated D-chiro-Inositol tracer was absorbed from the gastrointestinal tract. Rats chronically ingesting the chow diet consumed 921 (mol of total D-chiro-inositol/kg/day and excreted less than 5% of that amount in the stool and urine suggesting that the bulk was metabolized. However, even though urinary excretion of total D-chiro-i nositol was very small compared to intake, it declined further by over 98% in response to a chiro-inositol-deficient diet. Thus, urinary levels may reflect dietary intake. Recent reports have shown that D-chiro-inositol is found in certain putative insulin mediator fractions and that increased urinary excretion occurs in uncontroleld diabetes mellitus. Further work is needed to clarify the dietary and potential nutritive role of D-chiro-inositol.

含有D-手性肌醇的化合物普遍存在 实验动物饲料。 采用气相色谱-质谱 (GC 6 N HCl水解产物的纯化（100 μ g/MS），Purina罗登周5001含有 0.23重量%的D-手性肌醇。 主要来源是 苜蓿和大豆粉，而其他食物成分的含量低于 每克只有这些豆类的2%。 只有微量的L-手性肌醇 D-手性肌醇存在于水溶性非离子型 其中大多数形式显示为松醇， 1D-3-O-甲基-手性肌醇。 98%以上的D-手性肌醇 以Purina Rodent Chow 5001以及纯化的松醇和 氘代D-手性-肌醇示踪剂被吸收， 胃肠道 大鼠长期摄食普通饲料 消耗921（mol总D-手性肌醇/kg/天，排泄较少 超过粪便和尿液中含量5%，这表明 被代谢了 然而，尽管尿中排出的总 d-手性-i nositol与摄入量相比非常小， 对于缺乏手性肌醇的饮食，进一步增加了98%以上。 因此，尿液水平可以反映饮食摄入量。 最近的报告 表明在某些假定胰岛素中发现D-手性肌醇 介质分数和增加尿排泄发生在 无法控制的糖尿病 需要进一步开展工作，以澄清 D-手性肌醇的膳食和潜在营养作用。