DIGITAL DATA ACQUISITION SYSTEM

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 DIDAC系统现已在四台LANL流式细胞仪(两相敏感型流式细胞仪、三激光系统和快速混合动力学细胞仪)以及在NFCR工作人员讲授的年度课程中用于向实验室讲授仪器原理的演示流式细胞仪上常规使用。所有的DIDAC系统都运行在Linux 6.1操作系统下的Intel平台上。该系统具有革命性的能力，可以非常快速和复杂地控制我们所有细胞仪的操作，以及原始数据脉冲的四路分类和波形记录。DIDAC I的设计已经冻结，新一代数据采集系统的设计已经开始。DIDAC I使用了大约6年的技术，导致了物理上复杂的模块。商业流式细胞仪制造商对DIDAC I的一个反对意见是，每个检测器需要一个昂贵的大幅面电路板。借鉴在DIDAC I开发中获得的经验，第二代系统的设计正在顺利进行。原型电路板将在资源年结束前制造完成。DIDAC II是围绕一个商用数字信号处理器板构建的，该板具有可堆叠的总线格式。NFCR设计的包含模数转换器和相关电路的电路板将直接插入堆栈。四个探测器的电路板将适合4x6x6英寸的体积。使用可编程的DSP处理器可以通过软件实现信号处理的高度灵活性，这在DIDAC I中是不具备的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The DiDAC system is now in routine use on four LANL flow cytometers (two phase sensitive flow cytometers, the three-laser system and the rapid mix kinetics cytometer) as well as on the demonstration flow cytometer used to teach a lab on instrumentation principles during the Annual Course in flow cytometry taught in part by the NFCR staff. All the DiDAC systems are running on the Intel platform under the LINUX 6.1 operating system. This system has revolutionary capabilities for very fast and sophisticated control of the operation of all of our cytometers, as well as four-way sorting and waveform recording of the raw data pulses. The design of DiDAC I has been frozen and design of a new generation data acquisition system has begun. DiDAC I uses technologies that are ~6 years old resulting in physically complex modules. One objection of commercial flow cytometer manufacturers to DiDAC I has been that each detector requires an expensive, large format board. Drawing on the experience gained in the DiDAC I development, design of a second-generation system is well underway. The prototype circuit boards will be fabricated by the end of the Resource year. DiDAC II is built around a commercial digital signal processor board that has a stackable bus format. The NFCR designed boards that contain the analog to digital converters and associated circuitry will plug directly into the stack. Boards for four detectors will fit in a 4 x 6 x 6 inch volume. Use of a programmable DSP processor enables a high degree of flexibility in signal processing via software that is not available in DiDAC I.