DETERMINING THE FOLDED AND UNFOLDED STRUCTURE OF THE HIV-1 TAR RNA USING SAXS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 HIV-1的TAR RNA是RNA折叠中常见的结构基序，其构象特征与理解大多数结构RNA有关。 此外，TAR RNA在HIV的生命周期中起着至关重要的作用;对其结构的了解将为其功能的研究提供信息，并有助于寻找HIV药物。 本项目的目的是利用小角X射线散射（SAXS）测量反离子诱导的人类免疫缺陷病毒（HIV）反式激活反应元件（TAR）的折叠。 HIV TAR的结构将在其未折叠状态下以及在各种配体和反离子诱导的折叠构象中进行探测。 获得的结构将用于更好地了解HIV TAR的折叠景观，并为现有模型提供实验限制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. TAR RNA from HIV-1 takes the form of a common structural motif in RNA folding, its conformational properties are relevant to the understanding of most structured RNAs. Moreover, the TAR RNA plays a crucial role in the lifecycle of HIV; knowledge about its structure will inform studies of its function, and contribute to the search for an HIV drug. The goal of this project is to measure the counterion-induced folding of the transactivation response element (TAR) from human immunodeficiency virus (HIV) using small-angle x-ray scattering (SAXS). The structure of HIV TAR will be probed in its unfolded state, as well as in various ligand and counterion-induced folded conformations. The structures obtained will be used to better understand the folding landscape of HIV TAR and to provide experimental limits for existing models.