RAT MODEL OF ACUTE MYOCARDIAL INJURY WITH ISOPROTERENOL

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该项目重新启动： 目标 1.使用micro-CT评估异硬脂醇损伤对心脏功能的影响： 2.使用MR组织学评估损伤的性质和分布 研究设计 动物：12只Fisher 344雌性（140-160 gm）：6只对照组; 6只给药组 供试品：皮下注射32 mg/kg异戊烯醇 计算机断层扫描：定量终点-心脏总容量;左、右和间隔壁厚度; EDV、ESV、射血分数、每搏输出量、心输出量、短轴缩短分数 MR显微镜检查：组织学分辨率下受累区域的体积和位置（MR < 30微米） 最终报告：将编写一份最终报告，解决目标中阐述的问题。所有数据将通过体内显微镜中心的网络传输系统提供给GSK。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This project was reactivated: Goals 1. Assess the impact of isopeterinol injury on cardiac function using micro-CT: 2. Assess the nature and distribution of injury using MR histology Study Design Animals: 12 Fisher 344 femal (140-160 gm) : 6 control; 6 treated Test article: Isopeterenol @ 32 mg/kg subcutaneous injection CT: Quantitative endpoints - total cardiac volume; left, right, and septal wall thickness; EDV, ESV, ejection fraction, stroke volume, cardiac output, fractional shortening MR microscopy: Volume and location of affected areas @ histologic resolution (MR < 30 microns) Final Report: A final report will be composed addressing the questions articulated in the goals. All data will be made available to GSK via the web-based delivery system from the Center for In Vivo Microscopy.