CELLULAR DELIVERY OF RAT INTERVERTEBRAL DISC CELLS IN DISC DEGENERATION MODEL

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的目标是研究大鼠椎间盘（IVD）细胞在大鼠椎间盘退变模型中的递送。细胞将被递送至活体大鼠尾部（IVD）。 这些细胞将被荧光标记物（纳米颗粒，柯达光学成像仪）标记，以便我们确定植入细胞的具体位置。我们还旨在确定注射细胞的迁移模式，以帮助开发细胞递送至IVD的改进方法。在一段时间内跟踪植入细胞的能力将是对正在进行的改善基于细胞的递送方法的努力的重要补充。 细胞可以单独递送或与我们实验室开发的细胞递送载体（水凝胶支架）一起递送，以将植入的细胞维持在期望的位置。我们将计划一项预防性成像研究，其中植入的细胞将在24小时、3天、7天和14天成像，以确定标记的持续时间以及植入细胞的迁移模式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to investigate the delivery of rat intervertebral disc (IVD) cells in a rat model of disc degeneration. The cells will be delivered to live rat tails (IVD) percutaneously. The cells will be labelled with a fluorescent marker (nanoparticles, Kodak optical imager) to allow us to define the specific location of the implanted cells. We also aim to define the migratory patterns of the injected cells to assist in developing improved methods of cell delivery to IVD. The ability to track implanted cells over a time course will be a significant addition to ongoing efforts in improving cell based delivery methods. Cells may be delivered alone or with a cell delivery vehicle (hydrogel scaffold) developed in our laboratory to maintain the implanted cells in the desired locations. We will plan a longitutional imaging study where the implanted cells will be imaged at 24hrs, 3 days, 7 days and 14 days to determine both the duration of labeling as well as migratory patterns of implanted cells.