LONGITUDINAL 1H NMR SPECTROSCOPY OF MOUSE HD

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 本项目的目的是使用转基因小鼠模型研究亨廷顿病(HD)进展过程中发生的神经化学变化。亨廷顿氏病是一种遗传性神经退行性疾病，由某些大脑区域的脑细胞遗传程序性退化引起。含有缺陷人类基因的基因操纵的小鼠被用来模拟这种人类大脑疾病。在体内，高磁场下的~1H核磁共振波谱被用于对选定的小鼠大脑区域的代谢物进行非侵入性定量。这种方法的非侵入性特征使其能够纵向监测神经退行性变背后的生化变化。本项目的目的是从分子水平上更好地了解HD神经退行性变的机制和过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The purpose of this project is to investigate the neurochemical changes occurring during the progression of the Huntington's disease (HD) using transgenic mouse models. Huntington's disease is an inherited neurodegenerative disease resulting from genetically programmed degeneration of brain cells in certain brain regions. Genetically manipulated mice, which DNA contain the defected human gene, are used to mimic this human brain disease. In vivo 1H NMR spectroscopy at high magnetic fields is used for a non-invasive quantification of metabolites from selected mouse brain regions. Non-invasive character of this method enables a longitudinal monitoring of biochemical changes underlying the neurodegeneration. The aim of this project is a better understanding of the mechanisms and processes of the neurodegeneration in HD on the molecular level.