PROTEOMIC CHARACTERIZATION OF CEREBROSPINAL FLUID BY HIGH RESOLUTION LC-MS/MS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 这个NCRR合作项目的重点是人类脑脊液（CSF）的蛋白质组特征。 在临床医学中，CSF是常规采样，以帮助确定和监测大脑或脊髓中疾病的存在。 临床医生通常为此目的测量总蛋白浓度，因为对正常或疾病状态下CSF的蛋白质组成或蛋白质组知之甚少。 目前关于CSF的许多知识是基于2D凝胶分析技术，其在灵敏度和通量方面都受到严重限制。 本研究中提出的研究的总体目标有两个：（1）尽可能确定“正常”人CSF中蛋白质的含量和分布，为未来的定量、高灵敏度LC-MS研究创建准确的质量和时间（AMT）标签数据库;（2）评估CSF中蛋白质的含量和丰度如何在脑和脊髓的特定疾病中改变。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This NCRR collaborative project has focused on the proteome characterization of human cerebrospinal fluid (CSF). CSF is routinely sampled in clinical medicine to help determine and monitor the presence of illness in the brain or spinal cord. Clinicians usually measure the total protein concentration for this purpose since very little is known about the protein composition, or proteome, of CSF in normal or disease states. Much of the current knowledge about CSF is based upon 2D gel analysis techniques which are severely limited in both sensitivity and throughput. The overall goals of the research proposed in this study are twofold: (1) determine, as possible, the content and distribution of proteins in "normal" human CSF for the creation of an accurate mass and time (AMT) tag database for future quantitative, high sensitivity LC-MS studies; and (2) evaluate how the content and abundance of proteins in CSF are altered in specific diseases of the brain and spinal cord.