INTEGRIN ASSOCIATED PROTEINS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 整合素受体与细胞外基质(ECM)配体结合，选择性地连接到细胞内信号通路和肌动蛋白细胞骨架，调节黏附依赖的细胞过程。在癌症中，整合素对黏附的动态调节对于细胞与细胞外基质的黏附、细胞的增殖、侵袭和细胞的迁移至关重要。然而，整合素的细胞内下游效应对口腔癌的迁移、侵袭和转移具有重要作用，目前还不清楚。我们的目的是比较来自高转移和低转移口腔癌细胞的整合素相关复合体的组成。这项蛋白质组学研究将确定候选的整合素下游因子，这些因子要么与不同的整合素受体相关，要么与特定转移状态的整合素特异相关。未来的研究将描述已识别的因素是否调节侵袭和转移，以及参与调节这些转移表型的分子途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Integrin receptors bind to extracellular matrix (ECM) ligands and selectively connect to intracellular signaling pathways and the actin cytoskeleton to regulate adhesion-dependent cellular processes. In cancer, the dynamic regulation of adhesion by integrins is critical for cell attachment to ECM, cell proliferation, invasion and cell migration. However, the intracellular downstream effectors of integrins that are important for oral cancer migration, invasion and metastasis are not well understood. Our aim is to compare the composition of integrin associated complexes derived from highly and poorly metastatic oral carcinoma cells. This proteomics study will identify candidate integrin downstream factors that either associate with distinct integrin receptors or specifically associate with integrins in a particular metastatic state. Future studies will delineate whether the identified factors regulate invasion and metastasis, as well as the molecular pathways involved in regulating these metastatic phenotypes.