REGULATION OF MEMBRANE TRAFFICKING VIA PROTEIN-PROTEIN INTERACTIONS IN C ELEGA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 40多年来，人们一直热衷于了解蛋白质和细胞器运输到最终目的地的机制。最近，一些大的蛋白质复合体，如外囊和啤酒花复合体，被发现在膜运输的不同阶段起着关键的调节作用。以线虫为模型系统，我们有兴趣探索其他蛋白质-蛋白质相互作用如何影响膜运输机制的额外组件。使用RNA干扰可以快速地进一步探索使用这种方法确定的蛋白质的作用，这已被证明在线虫中工作得特别好

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Understanding the mechanisms by which proteins and organelles are transported to their final destination has been fervently pursued for more than 40 years. Recently, several large protein complexes such as the exocyst and the HOPS complex have been found to play critical roles in the regulation of different stages of membrane transport. Using C. elegans as a model system, we are interested in exploring how other protein-protein interactions may influence additional components of membrane trafficking machinery. Roles for proteins identified using this approach can quickly be further explored using RNA interference, which has been shown to work exceptionally well in C. elegans