Histology and Comparative Pathology Core

组织学和比较病理学(核心C)将提供U19 CMCR的四个项目 授予一个全面的组织病理学设施，帮助调查人员进行组织学分析 研究人员需要分析干细胞在改善辐射诱导结果中的作用 胃肠道综合征(RIGS)、辐射诱导的造血综合征(RIHS)以及 放射性皮肤综合征(RICS)。 该中心将就样品的制备和加工以及技术提供建议。 以及可用于不同研究的程序。核心将提供尸检和组织病理学 表型分析服务以及全方位的组织学服务 核心提供的服务将包括：方案设计、尸检，包括组织固定、组织 进行石蜡包埋、冰冻包埋、标准和特殊染色及 免疫组织化学分析。它还将包括利用组织样本的组织病理学评估 标准化的分析方法。 核心的KEV人员将是主任和组织学技术人员。导演将会是 负责监督核心的所有活动，就不同的方法和 程序，结果的解释，以及生成组织病理学报告和数据的解释。 技术人员将为手头的研究准备和处理适当的组织样本，执行一些 专门的技术和程序(如免疫组织化学和特殊染色)。 意义：适当的组织样品制备(固定、加工、修剪和染色)是 对本计划项目中的所有项目至关重要，因为它允许研究干细胞在体内的影响 关于辐射损伤后果的技术。

The Histology and Comparative Pathology (Core C) will provide the four projects of this U19 CMCR grant a comprehensive histopathology facility that will assist the investigators in the histological analysis required for the investigators to analyze the role of stem cells in improving the outcome of radiation induce gastrointestinal syndrome (RIGS), Radiation induced hematopoietic syndrome (RIHS), and Radiation-induced cutaneous syndrome (RICS). The core will provide advice on preparafion and processing of samples, as well as on the techniques and procedures available for the different studies. The Core will provide necropsy and histopathology services for phenotypic analysis along with a full range of histological services The services offered by the core will include: protocol design, necropsy, including tissue fixation, tissue proceeding to paraffin block, frozen embedding, standard and specialized stains and immunohistochemieal analyses. It will also include histopathological evaluafion of tissue samples utilizing a standardized method of analysis. The kev personnel of the core will be the director and the histology technical staff. The director will be responsible for supervising all the activities of the core, offering advice on the different methods and procedures, interpretafion of the results, and generating histopathology reports and interpretations of the data. The technical staff will prepare and process tissue samples as appropriate for the study at hand, perform some of the specialized techniques and procedures (e.g. immunohistochemistry and special stains). Significance: Appropriate tissue sample preparation (fixation, processing, trimming and staining) is essential to all projects in this Program Project as it allow the study of the in vivo impact of stem cell technologies on the outcome of radiation-induced injury.