Arf GTP-binding proteins and membrane traffic

我们继续评估Arf 6和高尔基体相关的Arfs如Arf 1在质膜上所起的作用。 GTP结合蛋白如Arf在GTP结合的活性形式和GDP结合的非活性形式之间循环。我们的发现，活性形式的Arf 6可以招募Arf鸟嘌呤核苷酸交换因子或GEFs的膜已形成的基础上，活性Arf 6（Arf 6-GTP）可以招募和激活其他Arf蛋白，如Arf 1，通常认为在高尔基复合体的功能。 我们发现Arf 1是由Src家族激酶和蛋白激酶C激活驱动的皮质肌动蛋白重排所必需的。我们正在确定哪些GEFs参与了这一过程，以及哪些GT3激活蛋白（GAP）参与了关闭Arf信号的过程。

We continue to evaluate the roles played by Arf6 and also Golgi-associated Arfs like Arf1 at the plasma membrane. GTP-binding proteins like Arf cycle between GTP-bound, active and GDP-bound, inactive forms. Our finding that active forms of Arf6 can recruit Arf guanine nucleotide exchange factors or GEFs to membranes has formed the basis of a model whereby active Arf6 (Arf6-GTP) can recruit and activate other Arf proteins like Arf1, typically thought to function at the Golgi complex. We find that Arf1 is required for cortical actin rearrangements driven by activation of Src family kinases and protein kinase C. We are identifying which GEFs are involved in this process and also which GTPase activating proteins (GAPs) that turn off the Arf signal are involved.