新的 hiPSC 工具用于以星形胶质细胞为中心的 ALS 治疗方法-猫眼课题宝

新的 hiPSC 工具用于以星形胶质细胞为中心的 ALS 治疗方法

批准号：

8293969

负责人：

KEVIN C EGGAN

金额：

$84.96万

依托单位：

JOHNS HOPKINS UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

2012

资助国家：

美国

项目状态：

已结题

起止时间：

2012-07-01 至 2014-06-30

来源：

https://reporter.nih.gov/project-details/8293969

关键词：

Activities of Daily Living Acute Affinity Amyotrophic Lateral Sclerosis Antibodies Astrocytes Binding Proteins Biochemical Biological Assay Cell Differentiation process Cell Line Cell Separation Cell Transplantation Cells Cervical Connexin 43 Coupling Cues Development Electrophysiology (science)Embryo Engraftment Evaluation Excitatory Amino Acid Transporter 2 Exhibits FK506 Family Fingers Fluo 4 Fluorescence Future GLAST Protein Generations Genes Genome Glial Fibrillary Acidic Protein Glutamate Transporter Glutamates Grant Gray unit of radiation dose Green Fluorescent Proteins Human Image Immunohistochemistry Immunosuppression In Vitro Individual Injection of therapeutic agent Insertional Mutagenesis Knock-in Mouse Laboratories Lactate Transporter Life Mediating Membrane Potentials Methods Monitor Morphology Mutation Neurons Phenotype Physiology Potassium Preclinical Drug Evaluation Property Proteins Protocols documentation Purinoceptor Reporter Reporter Genes Rest Rodent Schwann Cells Screening procedure Signal Transduction Site Slice Sorting - Cell Movement Specificity Spinal Cord Stem cells Technology Time Transplantation Whole-Cell Recordings Work Zinc Fingers aldehyde dehydrogenases aquaporin 4 base disease phenotype enhanced green fluorescent protein experience follow-up homologous recombination in vivo induced pluripotent stem cell injured juvenile animal nuclease promoter protein aggregate protein expression research study response tool transcription factor uptake water channel

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中文摘要

翻译

在最初的ALS iPS Go Grant提交时，还没有经过充分验证的从人类ES或IPS细胞系中产生星形胶质细胞的方案，尽管有来自啮齿动物细胞的方法。幸运的是，我们能够在Henderson实验室和Rothstein实验室开发出可靠的协议(图5A)。我们现在有一种在两个实验室中工作相同的协议，在实验室之间的区分方面效率非常相似。我们也已经能够在一些已经足够先进的细胞系中检测到真正的星形胶质细胞成熟的证据，这些细胞表达适当的星形胶质细胞特异性蛋白并具有功能谷氨酸转运(图5B-D)。

英文摘要

At the time of submission of the orginal ALS iPS Go Grant, there were no well-validated protocols for generating astroglia from human ES or IPS cell lines, although approaches from rodent cells existed. Fortunately, we were able to develop a reliable protocol in both the Henderson lab and the Rothstein labs (Figure 5A). We now have a protocol that works identically in both labs¿with very similar efficiency at differentiation between labs. We have also been able to detect evidence of true astroglial maturation in a number of lines that have advanced sufficiently and these express appropriate astroglial specific proteins and have functional glutamate transport (Figure 5B-D).

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