Develop Ponderomotive Phase Plate for Transmission Electron Microscopy of Biospec

描述（申请人提供）：介绍世界上第一台光学驱动透射电子显微镜相板的设计、建造和演示方案。这项技术，被称为ponderomomotive相位板，利用聚焦激光产生一个明确的电子相移，通过物镜的后焦平面。这种相移调制了仪器的对比度传递函数，使高对比度图像能够以最佳焦点记录，同时消除了较低空间频率下的对比度反转。该方法解决了与先前相位板设计相关的许多问题，并且是迄今为止提出的唯一一种有潜力提供令人难以置信的生物成像优势的方法，这种优势可以通过相位板获得，而不存在与Zernike和Boertsch相位板相关的重大问题。

DESCRIPTION (provided by applicant): Plans for the design, construction and demonstration of the world's first optically driven transmission electron microscope phase plate are described. This technology, known as a Ponderomotive Phase Plate, utilizes a focused laser to create a well defined phase shift of electrons passing through the back focal plane of the objective lens. This phase shift modulates the contrast transfer function of the instrument enabling high contrast images to be recorded at optimal focus while eliminating contrast reversals at lower spatial frequencies. This approach addresses and solves a multitude of issues associated with previous phase plate designs and is the only method proposed thus far that has the potential to deliver on the incredible biological imaging advantages that can be gained with a phase plate with none of the significant problems associated with Zernike and Boertsch phase plates. PUBLIC HEALTH RELEVANCE: IDES will provide an invaluable imaging tool for medical, pharmaceutical, and nano- technology researchers. The Pondermotive Phase Plate will enable the advancement of public health in areas as diverse as virology, drug discovery, drug delivery, cellular dynamics, and cell biology.