Resource for the Development of Biomedical Accelerator Mass Spectrometry (AMS)

国家加速器质谱资源（AMS）成立于1999年， 生物医学研究人员在探索基础放射性同位素的同时， 生物学的问题。在这次更新中，我们将通过开发一个完全集成的 HPLC AMS，以提高我们的合作者所需的代谢测量能力。我们将 开发研究生化途径和细胞过程直至单细胞水平的方法。 最后，我们将开发和验证AMS在人类翻译研究中的应用方法 这是协作者和服务用户需求不断增长的领域。在赠款的整个期限内， 我们将继续为研究界提供资源，包括为调查人员提供服务 熟悉AMS，对调查人员进行技术培训和资源传播。朝向 为了实现这些目标，我们的具体目标是： 1.）的人。通过直接耦合到分离仪器增加AMS的通量。 2.）的情况。通过结合分子特性和 对来自非常小的细胞、动物和人类样本的用于途径分析的确定分离株进行定量。 3.）第三章在取样材料中使用多种同位素示踪剂对生物系统进行定量。 4.）为协作和服务客户提供高通量精密定量。

The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing a fully integrated HPLC AMS to increase our capabilities for metabolic measurements which our collaborators require. We will develop methods to study biochemical pathways and cellular processes down to the level of the single cell. Finally we will develop and validate methods for the application of AMS in human translational research which is a growing area of demand by collaborators and service users. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Increased throughput of AMS through direct coupling to separatory instruments. 2.) Increase the value and information content of AMS measurements by combining molecular identities with quantitation of defined isolates for pathway analysis from very small cellular, animal, and human samples. 3.) Provide quantitation of biological systems using multiple isotopic tracers within sampled materials. 4.) Provide high throughput precision quantitation for collaborative and service clients.