Molecular Virology Core

马萨诸塞大学CFAR分子病毒学核心设施的总体目标是：（1）维护和持续监测BSL 3和BSL 2遏制设施的安全操作，这将支持一个安全和无生物危害的环境，用于致病性灵长类免疫缺陷病毒的工作;（2）为参与CFAR的调查人员提供资源和服务，以进行他们正在进行的病毒相关项目;以及（3）作为一个中心站点，促进马萨诸塞大学CFAR调查人员之间的合作。核心服务包括：（1）用于病毒的分离、滴定、表型和基因型表征的测定;（2）为原代T细胞、巨噬细胞和T细胞系提供集中的来源和储存库;以及（3）分子检测/诊断测定，包括用于病毒基因组的定性和定量RNA和DNA-PCR以及用于实时检测和定量HIV-1的最先进的基于LightCycler的PCR测定。分子病毒学核心设施，与马萨诸塞大学CFAR-临床和分子生物学核心设施一起，通过提供标准化和集中化的常规研究技术和试剂来维护马萨诸塞大学CFAR的使命;通过消除重复研究来减少开支。 努力&通过有效利用人员;以及促进新的合作项目和赠款。分子病毒学核心还致力于为CFAR调查人员和学生/研究员提供教育和培训机会，在BSL 3遏制设施内工作，开发新的分子病毒学检测，BSL 3认证和PCR认证。分子病毒学核心设施的详细信息，其位置， 功能，所提供的服务都可以在UMass CFAR分子病毒学核心网站（www.umassmed.edu/cfar/viroloavcore）上获得。

The overall objectives of UMass CFAR Molecular Virology Core facility are: (1) to maintain and continuously monitor the safety operations of a BSL3- and a BSL2-containment facility that will support a safe and biohazard-free environment for work with pathogenic primate immunodeficiency viruses; (2) to provide resources and services to participating CFAR investigators for their ongoing virus-related projects; and (3) to function as a central site where collaborations amongst UMass CFAR investigators is fostered. The Core services include: (1) assays for isolation, titration, phenotypic and genotypic characterization of virus; (2) providing a centralized source and repository for primary T-cells, macrophages & T-cell lines; and (3) molecular detection/diagnostic assays including qualitative and quantitative RNA and DNA-PCR for viral genomes & state of the art, cutting edge LightCycler-based PCR assays for real-time detection and quantitation of HIV-1. The Molecular Virology Core facility, in conjunction with UMass CFAR-Clinical and Molecular Biology Core facilities, uphold the mission of UMass CFAR by providing standardized and centralized routine research techniques and reagents; reducing expenditure by eliminating duplication of effort & by efficient use of personnel; and fostering new collaborative projects and grants. The Molecular Virology Core is also committed to provide educational & training opportunities to CFAR investigators and students/fellows in working within BSL3 containment facility, in developing new molecular virology assays, in BSL3 Certification, and in PCR Certification. Details of the Molecular Virology Core facility, its location, its functions, the services provided are all available on the UMass CFAR Molecular Virology Core website (www.umassmed.edu/cfar/viroloavcore).