Tomographic Fluorescent and Bioluminescent Optical Imaging

描述(由申请人提供)：本申请申请资助IVIS Spectrum，这是一种由卡利珀生命科学公司(马萨诸塞州霍普金顿)制造的用于临床前研究的活体小动物成像系统。IVIS光谱是一种先进的活体成像系统，用于对整个动物的深部组织生物发光和荧光源进行成像。它具有一套优化的高效滤光片和光谱分解算法，允许生物发光和荧光报告器跨蓝光至近红外波长成像。它还为荧光和生物发光记者提供单视图3D断层扫描，可以使用数字鼠标地图集在解剖学背景下进行分析。IVIS Spectrum采用定制的熔融石英1英寸CCD，视场在3.5厘米到25厘米之间，量子效率在500到700 nm之间达到85%，使用真正的f/1镜头。这些规格的组合提供了具有11个数量级的动态范围的高灵敏度生物发光成像。对于荧光成像，IVIS Spectrum可以使用透照或表观照明来照亮体内的荧光源。这种能力允许3D漫射荧光层析成像，从而可以确定源的位置和浓度。该仪器配备了窄带激发和发射过滤器，有助于显著减少自发荧光。与光谱分解工具相结合，这允许在同一动物中分离来自多个荧光记者的信号。IVIS光谱将作为宾夕法尼亚大学小动物成像设施(SAIF)的一部分提供给多个用户。它将被放置在新装修的2100平方英尺内。核心设施毗邻9.4T小型动物磁共振、微型PET和CT，以及动物超声系统，毗邻400个笼子动物的，配有先进的动物处理和手术室。IVIS Spectrum将为宾夕法尼亚大学的SAIF增加相当多的功能。 公共卫生相关性：该应用程序请求为IVIS光谱系统提供资金，用于对小鼠进行临床前光学成像。该系统的独特之处在于，它可以提供三维生物发光和荧光图像，其格式很容易与其他模式的图像共同配准。该系统将与现有的小动物核磁共振和超声波扫描仪相邻，用于开发和增强人类疾病小鼠模型的多模式成像。

DESCRIPTION (provided by applicant): This application requests funds for an IVIS Spectrum, an in vivo small animal imaging system for preclinical research use manufactured by Caliper Life Sciences (Hopkinton, MA). The IVIS Spectrum is an advanced in vivo imaging system for imaging of deep tissue bioluminescent and fluorescent sources in whole animals. It features an optimized set of high efficiency filters and spectral un-mixing algorithms that allow imaging of bioluminescent and fluorescent reporters across the blue to near infrared wavelengths. It also offers single- view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using a Digital Mouse Atlas. The IVIS Spectrum incorporates a custom fused silica 1 inch CCD with a FOV between 3.5 cm and 25 cm, a quantum efficiency of 85% between 500-700nm and a true f/1 lens. The combination of these specifications provides for highly sensitive bioluminescent imaging with a dynamic range of 11 orders of magnitude. For fluorescence imaging, the IVIS Spectrum can use either trans-illumination or epi-illumination to illuminate in vivo fluorescent sources. This capability allows 3D diffuse fluorescence tomography such that both source localization and concentration can be determined. The instrument is equipped with narrow band excitation and emission filters that assist in significantly reducing autofluorescence. In combination with the spectral unmixing tools, this allows the separation of signals from multiple fluorescent reporters within the same animal. The IVIS Spectrum will be available to multiple users as part of the Small Animal Imaging Facility (SAIF) at the University of Pennsylvania. It will be placed in the newly renovated 2,100 sq ft. Core Facility adjacent to the 9.4 T small animal MR, micro-PET and CT, and animal ultrasound systems, adjacent to a 400 cage animal vivarium with advanced animal handling and surgery rooms. The IVIS Spectrum will add considerable functionality to the SAIF at the University of Pennsylvania. PUBLIC HEALTH RELEVANCE: This application requests funds for an IVIS Spectrum system for preclinical optical imaging of mice. The system is unique in that it can provide three-dimensional bioluminescence and fluorescence images in a format that is easily co-registered with images from other modalities. The system will be located adjacent to existing small animal MRI and ultrasound scanners and used to develop and enhance multi-modality imaging in mouse models of human disease.