Acquisition of a Transmission Electron Microscope to Reactivate Facility

申请材料(申请人提供)：申请购买JEOL JEM-1400透射电子显微镜，以支持美国国立卫生研究院资助的迈阿密大学米勒医学院研究小组的研究项目。这些研究人员中的一些人最近搬到了这个机构，对瞬变电磁有很大的需求，而现有的仪器无法满足这一需求。当需要高分辨率时，瞬变显微镜是绝对必要的，这是标准共聚焦显微镜无法实现的。小组中的所有调查人员都需要需要在 只有通过透射电子显微镜才能实现的几个纳米。小组中的所有调查人员 在NIH资助的研究中拥有丰富的电子显微镜经验。预计这一应用中核心调查人员群体的需求将占仪器时间的92%。预计将有更多新的调查人员加入核心小组，并提出一项计划，对他们进行培训，并向他们提供已在校园中的训练有素的EM技术员的专家支持。该设施将设在已有的完全翻新的空间内，由两名全职教员(PI和另一名主要用户)指导和共同指导，并由上述技术经理监督，他们将主要负责维护仪器和培训新用户。已经组织了一个咨询委员会来监督该文书的使用和维护。咨询委员会制定了使用和公平分享该文书的政策，并制定了长期维持该文书的计划。坚定的机构承诺为核心设施和仪器设备提供持续的支持，包括空间翻新、对未来服务合同的100%支持和管理人员工资的一小部分。现有的瞬变电磁仪已经大量使用且非常过时，无法满足主要用户群体的瞬变电磁法需求。校园里的仪器都没有提供数字成像。改善对这项技术的获取将极大地增强NIH资助的用户群体和校园内可能的未来用户的项目。总而言之，这个应用程序寻求资金来取代已经使用了22年的废弃的共享资源。

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase a Jeol JEM-1400 Transmission Electron Microscope (TEM), to support the research projects of a group of NIH-funded investigators at the University of Miami Miller School Of Medicine. Some of these investigators have recently moved to this institution and have heavy needs for TEM that cannot be fulfilled by the available instruments. TEM is absolutely essential when high-resolution is needed, that cannot be achieved with standard confocal microscopy. All the investigators in the group need morphological readouts that require resolution within a few nanometers that can only be achieved by TEM. All the investigators in the group have had extensive experience with electron microscopy in their NIH-funded research. It is estimated that the needs of the core group of investigators in this application will account for 92% of the instrument time. A number of new additional investigators is expected to join the core group and a plan is put forth to train them and provide them with the expert support f a well-trained EM technician already in campus. The facility will be housed in fully renovated space already available and will be directed and co-directed by two full-time faculty members (the PI and another major user) and supervised by the technical manager mentioned above, who will have primary responsibility for maintaining the instrument and training new users. An advisory committee has been organized to oversee the use and maintenance of the instrument. Policies for the use and equitable sharing of the instrument, and a plan for its long-term maintenance have been established by the advisory committee. Strong institutional commitment provides ongoing support for the core facility and the instrumentation, and includes space renovation, 100% support for future service contracts and a fraction of the salary of the manager. The TEM needs of the primary user group cannot be satisfied by existing TEM instruments that are already heavily used and very outdated. None of the instruments in campus provide digital imaging. Improved access to this technology will greatly enhance the NIH-funded projects of the user group and possible future users in campus. In summary, this application seeks funding to replace a defunct shared resource that was in use for over 22 years.