BioMark

描述(由申请人提供)： 项目摘要和摘要斯克里普斯研究所的这项提案旨在从Fluidigm公司购买一台BioMark仪器。该仪器将致力于在转录水平上研究单个细胞，并响应TSRI科学家日益增长的需求。该研究所致力于全力支持这一由免疫学和微生物科学系提出的申请。运营所需的必要资源和人员将在核心董事Steven Head的监督下，通过将新仪器安置在TSRI的DNA核心内进行投入。我们对BioMark机器的需求是因为迫切需要为我们的机构带来一种新技术，这种技术将允许目前无法在现场进行的实验，并将提高我们的产品质量。这种微流控仪器具有通用性的巨大优势，因为它可以用于实时聚合酶链式反应、数字聚合酶链式反应、基因分型和测序。关于单细胞分析，我们找不到任何具有竞争力的基因组硬件公司提供的类似设备。在免疫学方面，随着社区现在意识到细胞群体的异质性，研究单细胞的需求在过去几年里呈爆炸式增长。我们通过分别使用标记抗原和标记MHC多聚体的荧光激活细胞分选来分离特定的单个B和T细胞的能力，极大地促进了这种观察免疫反应的新方法。目前的障碍是我们从孤立的单个细胞中提取多参数信息的能力有限。同样，神经生物学家想要询问单个神经元，癌症生物学家想要检查肿瘤的异质性。该仪器将被安置在TSRI DNA核心设施中并由其操作。对于免疫学应用，研究人员将以成本价获得整个引物集合。事实上，免疫学应用所需的引物组的收集在小鼠和人身上都是非常标准化的，很容易分类。作为一项核心工具，生物标志将在核心主任及其咨询委员会的监督下提供给校园内的所有调查人员。核心将能够提供基本培训，尽管在使用该仪器之前，将高度鼓励对制造商进行正式培训。预订将在核心董事的监督下在线进行。该仪器将根据由成本回收计划支付的维护合同。

DESCRIPTION (provided by applicant): Project Summary and Abstract This proposal by the Scripps Research Institute is aimed at purchasing a Biomark instrument from Fluidigm. This instrument will be dedicated to studying single cells at the transcriptional and respond to an increasing demand from the scientists of TSRI. The Institute is committed to fully support this application that stems from the Department of Immunology and Microbial Science. The necessary resources and personnel needed for operation will committed through housing the new instrument within the DNA Core of TSRI under the supervision of the Core director, Steven Head. Our request for a Biomark machine is motivated by the urgent need to bring to our institution a new technology that will allow experiments that cannot currently be carried out on-site and that will add to the quality of our output. This microfluidic machine has the huge advantage of versatility as it can be used for real-time PCR, digital PCR, genotyping, and sequencing. With respect to single cell analysis, we could not find comparable pieces of equipment from any of the competitive genomics hardware companies. In immunology, the need to study single cells has exploded over the past few years as the community has now realized of the heterogeneity of cell populations. This new way of looking at the immune response has been greatly facilitated by our capacity to isolate specific single B and T cells by fluorescence activated cell sorting using labeled antigens and labeled MHC multimers, respectively. The current hurdle is the limited ability that we have to extract multiparametric information from the isolated single cell. Similarly, neurobiologists want to interrogate single neurons and cancer biologist want to examine tumor heterogeneity. The instrument will be housed in and operated by the TSRI DNA Core Facility. For immunological applications the entire collection of primers will be available at cost to investigators. Indeed, te collection of primer sets needed for immunological applications is very standardized in both mouse and man and can be easily categorized. As a core instrument, the Biomark will be available to all investigators on campus under the supervision of the core director and its advisory committee. The core will be capable of providing basic training although formal manufacturer training will be highly encouraged before using the instrument. Booking will be on-line and under the supervision of the core director. The instrument will be under a maintenance contract paid by a cost-recovery program.