Equipment for Gross Hall Vivarium

描述(由申请人提供)：本申请申请资金，以装备一个饲养中心在一个新的设施，格罗斯霍尔动物资源中心(GHARC)，由HHS CO6 ARRA赠款，编号1C06RR030131-01资助，并支持一组国家卫生研究院资助的研究人员在加州大学欧文分校(UCI)。该项目将实现UCI动物计划的具体目标，并解决目前预算中GHARC的不足。拟建的繁育中心将由两个固定套房组成，每个套房40‘x23’=920 SF x 2套房=1840 SF，将位于格罗斯大厅动物资源中心(GHARC)的围栏内，该中心位于UCI园区最新的研究大楼Sue and Bill Gross Hall的地下室，净可分配平方英尺(23,385平方英尺)。该育种中心将支持饲养和饲养小鼠，包括有价值的转基因小鼠。一个例子：为了更好地模拟阿尔茨海默病(AD)的神经病理，英国皇家大学神经生物学和行为学教授弗兰克·拉费拉创造了一个AD的三重转基因模型(3xTg-AD)。LaFerla没有跨越独立的品系，而是将两个转基因(ssAPP和tau)显微注射到纯合PS1M146V敲击小鼠的单细胞胚胎中，产生具有相同遗传背景的小鼠。这些小鼠被广泛用于AD研究，并出口到其他研究机构。在UCI，有价值的转基因小鼠繁育者，如3xTg-AD，被安置在育种中心，采用严格的生物安全标准：广泛的病原体排除名单、强制性的再衍生、有限的研究人员准入、适当的个人防护装备(PPE)等。所需设备包括十二(12)个双面笼架(140个笼架/架)、四(4)个动物转运站和两(2)个二级A型生物安全柜。在UCI，有价值的转基因小鼠品系创始人只要有可能就被安置在专门的育种中心--生物科学的McGaugh Hall和BRC的Hewitt Hall。这些育种中心使用严格的生物安全标准：广泛的病原体排除名单、强制重新衍生、有限的研究人员访问权限和适当的个人防护措施。随着GHARC中可容纳7,280个鼠笼的容量的增加，休伊特霍尔的单一繁育中心不足以为Gross-Gillesbie-Hewitt BRC复合体提供实验小鼠。位于GHARC的育种中心将支持18名研究人员，NIH的资金超过1080万美元，还有490万美元悬而未决。笼子和设备将主要支持一组由PHS资助的基础和翻译研究人员在再生医学、阿尔茨海默病、癌症、糖尿病和其他代谢疾病、黄斑变性、视网膜色素变性、疱疹相关失明、免疫学、脊髓损伤和中风方面使用的种畜。格罗斯大厅是GHARC和拟建育种中心的所在地，已获得LEED白金认证，拟建育种中心设施将使用“绿色”笼架(能效提高63%)；可变风量(VAV)房间暖通空调，这是校园第一个隔间；以及一个高效的干热灭菌器。

DESCRIPTION (provided by applicant): This application requests funds to equip a Breeding Center in holding space in a new facility, Gross Hall Animal Resource Center (GHARC), funded by an HHS CO6 ARRA grant NIH Grant no. 1C06RR030131-01 and in support of a group of NIH-funded researchers at the University of California, Irvine (UCI). The project would achieve specific aims for the UCI animal program, and addresses deficiencies in the GHARC as currently budgeted. The proposed Breeding Center would consist of two holding suites, each 40'x23' = 920 sf x 2 suites = 1840 sf total, and would be located inside the barrier in the Gross Hall Animal Resource Center (GHARC), which occupies 17,433 net assignable square feet (23,385 gross square feet) in the basement of Sue and Bill Gross Hall, the newest research building on the UCI campus. The Breeding Center will support breeding and holding of mice, including valuable genetically modified mice. One example: to better model Alzheimer's Disease (AD) neuropathology, Frank LaFerla, Chancellor's Professor of Neurobiology and Behavior, created a triple transgenic model of AD (3xTg-AD). Rather than crossing independent lines, LaFerla microinjected two transgenes (ssAPP and tau) into single- cell embryos from homozygous PS1M146V knockin mice, generating mice with the same genetic background. These mice are used extensively in AD research and exported to other research institutions. At UCI, valuable genetically altered mouse breeders such as the 3xTg-AD are housed in Breeding Centers using strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, appropriate personal protective equipment (PPE), etc. The two Breeding Center holding/procedure suites consist of an Anteroom, Procedure room, and two Holding rooms. Requested equipment includes twelve (12) double sided cage racks (140 cages/rack), four (4) animal transfer stations, and two (2) Class 2 Type A biological safety cabinets. At UCI, valuable genetically altered mouse line founders are housed whenever possible in specialized Breeding Centers-McGaugh Hall in Biological Sciences and Hewitt Hall in the BRC. These Breeding Centers use strict biosecurity standards: broad pathogen exclusion list, mandatory rederivation, limited research staff access, and appropriate PPE. With the addition of capacity for 7,280 mouse cages in the GHARC, the single Breeding Center in Hewitt Hall is insufficient to provide experimental mice for the Gross-Gillespie-Hewitt BRC complex. The Breeding Center in the GHARC will support 18 researchers with NIH funding in excess of $10.8 million, and $4.9 million pending. The caging and equipment will support primarily breeding stock used by a group of PHS-funded basic and translational researchers in regenerative medicine, Alzheimer's disease, cancer, diabetes and other metabolic disorders, macular degeneration, retinitis pigmentosa, herpes-related blindness, immunology, spinal cord injury, and stroke. Gross Hall, site of the GHARC and proposed Breeding Center, has received LEED Platinum certification, and the proposed Breeding Center Facility would use "green" cage racks (63% more energy efficient); Variable Air Volume (VAV) room HVAC, the campus's first in a vivarium; and an efficient dry heat sterilizer.