Voltage-sensitive dye imaging setup

描述（由申请人提供）：我们请求资助在埃默里大学生物系建立一个电压敏感染料（VSD）成像装置。这一设置将大大提高神经科学系的研究能力，并得到NIH的积极资助（Jaeger，Calabrese，Liu和Prinz博士）。私家侦探(Dr. Jaeger）在基底神经节和小脑网络的研究方面有着悠久的历史。将这种分析扩展到基底神经节和小脑反馈回路引起的皮质活动调制水平将是创新的、有影响力的和及时的。VSD成像小鼠在体皮层活动将是一个重要的工具，以确定时间和空间的影响，基底神经节和小脑激活皮层活动。同样，刘博士的研究关注的是皮层活动模式，尽管是在小鼠听觉回路上的社交超声信号之后的激活。 VSD成像将使这项工作朝着更好地理解初级听觉皮层以外的空间和时间信号流的方向发展。对于Jaeger博士和Liu博士来说，这项技术将特别及时地很快实施，因为光遗传学刺激技术和遗传电压敏感指标的发展将使我们能够解决重要问题，并对新发现产生预期的高影响。Calabrese和Prinz博士的工作分别探索了无脊椎动物模式生成回路在水蛭心跳回路和螃蟹/龙虾胃神经节中的功能。然而，类似于皮质动力学的空间动力学的问题，这些神经节包括一个空间网络在各自的神经节与复杂的活动模式，可以卓有成效地调查电压敏感染料成像技术。我们要求的设备将允许我们通过在体内成像装置或Olympus BX 50显微镜上安装最先进的双CMOS相机系统，灵活地在啮齿动物体内（10 × 10 mm感兴趣区域）和无脊椎动物神经节（1 × 1 mm感兴趣区域）所需的空间尺度上进行实验。两种设置将共享操纵器和放大器，以进行同步电生理记录。主要研究者将提供此设置所需的大量基本设备（Olympus BX 50显微镜、隔振台、记录放大器），部门将提供专用房间。生物学。要求的核心项目是双摄像头CMOS系统，沿着必要的光学设备（分束器、软件等）和辅助硬件。从获得这种设置的期望是，创新和及时的方法将提供给主要研究人员的既定研究计划，并允许初级教师的发展以及。健康影响也是高度相关的，因为Jaeger实验室的研究直接应用于建立帕金森病机制，Liu实验室的工作与自闭症的研究有关，而Calabrese和Prinz实验室的工作证明了神经调节和稳态的基本机制，这些机制是理解病理性大脑活动如何作为正常动力学的适应不良而出现的基础。

DESCRIPTION (provided by applicant): We request funding to establish a voltage-sensitive dye (VSD) imaging setup in the Department of Biology at Emory University. This setup will greatly enhance the research capability of the neuroscience faculty in the Department with active NIH funding (Drs. Jaeger, Calabrese, Liu and Prinz). The P.I. (Dr. Jaeger) has a long track record in the research of basal ganglia and cerebellar networks. To extend this analysis to the level of cortical activity modulation due to basal ganglia and cerebellar feedback loops would be innovative, impactful, and timely. VSD imaging of mouse cortical activity in vivo will be a crucial tool to determine the temporal and spatial effects of basal ganglia and cerebellar activation on cortical activity. Similarly, the research of Dr. Liu is concerned with cortical actiity patterns, albeit the activation following social ultrasound signaling in mice on auditory circuits. VSD imaging would allow this work to progress towards a better understanding of spatial and temporal signal flow beyond primary auditory cortex. For both Dr Jaeger and Dr. Liu this technology would be particularly timely to implement soon, as the development of optogenetic stimulation techniques and genetic voltage-sensitive indicators will allow us to address important questions with an expected high impact in novel findings. The work of Drs. Calabrese and Prinz explores the function of invertebrate pattern generation circuits in the leech heartbeat circuit and crab/lobster stomatogastric ganglion, respectively. However, similarly to the question of spatial dynamics of cortical dynamics, these ganglia consist of a spatial network in the respective ganglia with complex activity patterns that can be fruitfully surveyed by voltage-sensitive dye imaging techniques. The equipment we request will allow us to flexibly conduct experiments on the spatial scale required in rodents in vivo (10x10 mm area of interest) and invertebrate ganglia (1x1 mm area of interest) by mounting a state of the art dual CMOS camera system either on an in vivo imaging setup or on an Olympus BX50 microscope. Both setups will share manipulators and amplifiers for simultaneous electrophysiological recordings. A substantial amount of the basic equipment (Olympus BX50 microscope, vibration isolation table, recording amplifiers) needed for this setup will be contributed by the major investigators, and a dedicated room is made available by the Dept. of Biology. The core item requested is the dual camera CMOS system, along with the necessary optical equipment (beam splitter, software, etc), and auxiliary hardware. The expectation from obtaining this setup is that innovative and timely methods will be available to the established research programs of the major investigators, and allow the development of junior faculty as well. The health impact is also highly relevant, in that the research by the Jaeger lab is directly applied to establishing Parkinson's disease mechanisms, work in the Liu lab is relevant to the study of autism, and work in the Calabrese and Prinz labs demonstrate fundamental mechanisms of neuromodulation and homeostasis that underlie understanding of how pathological brain activity may arise as a maladaptation of normal dynamics.