Microbial interactions in otitis media

项目总结 本项目旨在明确多菌感染如何影响细菌性中耳炎。嗜血杆菌 流感病毒(Hi)、肺炎链球菌(Sp)和卡他莫拉菌(Mcat)是优势菌株 来自OM患者，大量证据表明OM通常涉及这些因素的组合 有机体。我们假设细菌物种之间的相互作用可以影响OM疾病 参数包括细菌持久性、对清除和抗生素治疗的抵抗力，以及 宿主炎症反应的启动、进展和严重程度。为了解决这个问题 假设我们将完成以下具体目标： 具体目标1：确定引起OM的细菌病原体之间的相互作用。 具体目的2.探讨不同种间关系对实验性OM的影响。 具体目的3.评价多菌感染对抗生素耐药性的影响。 具体目的4.建立鼓室造口术患者合并感染的发生率。 我们目前关于细菌致病机制的大部分知识都来自于对感染的研究 可能不能完全代表实际患者感染发生情况的纯培养。这份提案提出了 了解栖息在同一生态位中的多种病原体如何影响一种病原体的机会 另一种是在对持续感染至关重要的生物膜社区。

PROJECT SUMMARY This project aims to define how polymicrobial infection influences bacterial otitis media. Haemophilus influenzae (Hi), Streptococcus pneumoniae (Sp), and Moraxella catarrhalis (Mcat) are the predominant isolates from patients with OM, and a wealth of evidence indicates that OM often involves combinations of these organisms. We hypothesize that interactions between bacterial species can impact OM disease parameters that include bacterial persistence, resistance to clearance and antibiotic treatment, and the initiation, progression and severity of the host inflammatory response. In order to address this hypothesis we will complete the following Specific Aims: Specific Aim 1: To define interactions among bacterial pathogens causing OM. Specific Aim 2. To ask how different interspecies relationships affect experimental OM. Specific Aim 3. To evaluate impact of polymicrobial infection on antibiotic resistance. Specific Aim 4. To establish the incidence of coinfection in patients undergoing tympanostomy. Most of our current knowledge about bacterial pathogenesis is derived from infection studies using pure cultures that may not fully represent how infections occur in actual patients. This proposal presents an opportunity to learn how multiple pathogens inhabiting the same ecological niche influence one another in biofilm communities that are critical to persistent infections.