Adaptive Optics Retinal Imaging in a Clinical Setting Using Long Stroke Wavefront

描述(由申请人提供)：这个SBIR第二阶段竞争性更新项目的主要目标是证明项目团队在第一阶段和第二阶段工作中设计和建造的独特的自适应光学扫描激光眼底镜(AOSLO)的临床相关性。它的特点是闭环式声光控制，可以自动补偿眼睛的像差，产生几乎受衍射限制的共焦视网膜图像。这在视网膜成像中尤其有用，因为使用AO(~2？m)和不使用AO(~10？m)的可分辨特征大小的差异对应于包含视网膜许多最重要特征的大小的范围：视锥感光细胞、神经纤维细胞、红细胞、白细胞、毛细血管和视网膜色素上皮细胞。因此，AOSLO应该有助于更好地了解视网膜功能和疾病。AOSLO原型包含一个光学倍增器和定制开发的长行程可变形镜，用于补偿更高阶像差。这些独特的部件使像差补偿成像适用于90%的临床受试者，而不需要使用额外的试验镜头。在第二阶段的工作中，AOSLO被证明实现了出色的视网膜图像分辨率和操作特性，使其非常适合临床使用。它使用长波长和低强度光源照明，因此可以在不放大光瞳的情况下对许多对象成像。它还包括一个波束控制系统，允许临床医生在不需要连续的受试者重新固定的情况下将成像的视野平移到整个视网膜。使用这种AOSLO平台技术将在临床环境中探索的创新成像技术包括个体血细胞跟踪、血流动力学成像和光感受器的蒙太奇映射。该项目将包括在成像、人体工程学和原型AOSLO的软件控制方面开发临床灵感增强功能。BMC将与一家临床网站合作，评估AOSLO的临床实用性。乔斯林糖尿病中心Beetham眼科研究所(BEI)将进行观察性先导研究，BEI研究的临床目的将是评估AOSLO视网膜图像作为临床上有用的视力替代品的能力 研究中心受累的糖尿病黄斑水肿患者的AOSLO图像，并评估量化的AOSLO图像指标与糖尿病视网膜病变的视网膜病变状态的相关性。

DESCRIPTION (provided by applicant): The principal objective for this SBIR Phase II Competitive Renewal project is to demonstrate clinical relevance of a unique Adaptive Optics Scanning Laser Ophthalmoscope (AOSLO) that the project team designed and built in Phase I and Phase II work. It features closed-loop AO control that automatically compensates for aberrations of the eye, yielding nearly diffraction-limited confocal retinal images. This is particularly useful in retinal imaging, since the difference in resolvable feature size with AO (~2¿m) and without AO (~10¿m) corresponds to a range that includes the size of many of the retina's most important features: cone photoreceptors, nerve fiber cells, erythrocytes, leukocytes, capillaries, and retinal pigment epithelial cells. As a result, the AOSLO should lead to a better understanding of retinal function and disease. The prototype AOSLO incorporates an optical doubler and custom-developed long-stroke deformable mirror for compensating higher order aberrations. Those unique components allow aberration- compensated imaging suitable for 90% of clinical subjects without the use of added trial lenses. In Phase II work, the AOSLO was demonstrated to achieve exceptional retinal image resolution and operational characteristics that make it well suited for clinical use. It uses long wavelength and low intensit source illumination, so that many subjects can be imaged without pupil dilation. It also includes a beam steering system that allows a clinician to translate the imaged field across the retina without requiring continual subject refixations. Innovative imaging techniques that will be explored in the clinical settings using this AOSLO platform technology include individual blood cell tracking, hemodynamic imaging, and montage mapping of photoreceptors. The project will include development of clinically inspired enhancements in imaging, ergonomics, and software control of the prototype AOSLO. BMC will collaborate with a clinical site in an evaluation of the clinical usefulness of the AOSLO. Observational pilot studies will be conducted at Beetham Eye Institute (BEI) of the Joslin Diabetes Center Clinical aims for the BEI study will be to evaluate the capability of AOSLO retinal images to serve as a clinically useful surrogates for visual acuity in eyes with center-involved diabetic macular edema, and to evaluate correlations between quantified AOSLO image metrics and the state of retinal disease in diabetic retinopathy.