Tissue Engineering of New Hair Formation

描述（由申请人提供）：本提案的目的是开发可以从解离的干细胞形成新毛发的重建皮肤。利用分离的多潜能真皮和表皮干细胞产生毛囊的研究已取得进展。然而，在临床应用取得重大进展之前，需要填补夫妇差距。最近，我们已经将Lichti和Stenn先前的测定法显著改进为具有临床上适当外观和高通量测定潜力的更简单的毛发形成测定法。我们的一般假设是，多能皮肤干细胞可以通过分子重编程产生，并且毛发原基的排列和图案可以通过环境条件进行调节。利用新开发的“平面毛发形成程序”，我们能够运用我们在羽毛图案形成方面的专业知识来调节毛发原基的数量和大小。我们还将运用我们最近的经验，对毛干细胞的宏观环境调控，以提高头发的形成效率。最近在其他器官中分子重组的成功激发了人们的兴奋。这种简单的检测方法将使我们能够进行高通量分析，以识别对将成人皮肤细胞重新编程为形成毛发的真皮和表皮细胞重要的分子或分子组合。这项技术的发展将为那些遭受严重创伤和脱发的人带来新的希望。 公共卫生相关性：目前，在美国每年有超过100，000名患者因烧伤而接受治疗，并且烧伤可能对皮肤上的可见区域造成持久的美容和功能缺陷。在这里，我们建议开发一种新的平面毛发形成方案，该方案整合了我们的母基金和其他毛发研究的进展，可能会给那些遭受严重创伤和脱发的人带来新的希望。

DESCRIPTION (provided by applicant): The objective of this proposal is to develop reconstituted skin that can form new hairs from dissociated stem cells. Progress has been made in producing hair follicles using dissociated multi-potential dermal and epidermal stem cells. However, couple gaps need to be filled before significant progress in clinical applications can be made. Recently, we have improved significantly the previous assays by Lichti and Stenn into a simpler hair forming assay with clinically proper appearance and potential for a high throughput assay. Our general hypothesis is that multi-potent skin stem cells can be generated by molecular reprogramming, and that arrangement and patterning of hair primordia can be regulated by environmental conditions. With the newly developed "planar hair forming procedure", we are able to apply our expertise in feather pattern formation to regulate the number and size of hair primordia. We will also apply our recent experience on macro-environment regulation of hair stem cells to improve the efficiency of hair formation. Recent success of molecular re-programmig in other organs has inspired excitement. This simple assay will allow us to do high throughput analyses to identify molecules or combinations of molecules important to reprogram adult skin cells into hair forming dermal and epidermal cells. The development of this technology will bring new hope to those who suffer from severe wounds and alopecia. PUBLIC HEALTH RELEVANCE: Currently, over 100,000 patients are treated for burn injury in the United States per year, and burns can cause lasting cosmetic and functional defects to visible areas on the skin. Here we propose to develop a novel planar hair forming protocol which integrates progress from our parent grant and others hair research and may bring new hope to those who suffer from severe wounds, and alopecia.