Mouse Imaging and Pathology

项目摘要（见说明）：小鼠病理学和成像核心一直是甲状腺癌P01补助金的重要组成部分，支持所有四个研究项目使用小鼠模型。核心的一个主要目标是提供小鼠病理学方面的专业知识，特别关注甲状腺病理学的详细方面，包括小鼠甲状腺癌的病理生物学。病理学支持将由兽医解剖学和临床病理学家提供，他们是美国兽医病理学家学院的文凭。比较甲状腺病理学方面的专业知识将使研究项目中的小鼠模型得到适当的解释和评价，并与人类甲状腺癌进行比较。小鼠临床病理学服务还包括Krista La Perie博士实验室的全方位血液学和临床化学分析仪，可以适当地分析小鼠的少量血液。这种结构将为研究项目提供卓越和连续的服务，并允许在该项目中使用的各种小鼠模型中观察到的病理变化的一致性和高水平整合。此外，该核心将提供最先进的成像服务，并在甲状腺分析的先进模式方面拥有先进的专业知识，例如使用高分辨率的解剖成像，3D小鼠甲状腺超声检查和使用集成microCT/microSPECT/microPET的功能成像。后一种成像能够分析小鼠中与临床上用于甲状腺癌患者的放射性示踪剂相同的放射性示踪剂，包括用于评估碘摄取和保留的放射性碘，以及用于评估代谢活性的氟脱氧葡萄糖。通过核心可用的其他成像模式包括数字Faxitron高分辨率X射线摄影，高分辨率小动物MRI，以及整个动物或器官生物发光和荧光成像。研究者可获得的其他服务包括（1）小鼠甲状腺肿瘤原代培养的技术专业知识和支持;（2）转移研究的支持，包括将肿瘤组织原位植入甲状腺床和将肿瘤细胞注射到尾静脉和/或左心室以诱导转移性疾病;和（3）支持开发用于新小鼠模型开发的新转基因和靶向等位基因。设施将包括尸检设施、自动免疫染色仪、显微镜设施（包括带视频输出的会议用多头显微镜）、专用生物发光和荧光体内成像仪器、专用小动物高分辨率/高频超声设备、数字大体和显微摄影设施、定量图像分析软件以及小鼠手术和放射学设施。由于该项目中的科学分布在各种模型和物理位置中，因此可以使用单个核心来协调、分析和比较数据，这将提供一个重要的资源，并显着提高研究数据的质量和有效性。

PROJECT SUMMARY (See instructions): The Mouse Pathology and Imaging Core has been an essential part of the Thyroid Cancer P01 grant, supporting the use of mouse models for all four of the research projects. A major goal of the Core will be to provide expertise in mouse pathology with a specific focus on detailed aspects of thyroid gland pathology, including the pathobiology of mouse thyroid carcinoma. Pathology support will be provided by veterinary anatomic and clinical pathologists that are Diplomates of the American College of Veterinary Pathologists. Expertise in comparative thyroid gland pathology will enable appropriate interpretation and evaluation of the mouse models in the research projects and comparison to human thyroid cancer. The mouse clinical pathology service also includes full-service hematology and clinical chemistry analyzers in the laboratory of Dr. Krista La Perie that can appropriately analyze small blood volumes from mice. This structure will provide excellence and continuity of service for the research projects, and allow consistency and high level integration of pathologic changes observed in the various mouse models used in this project. Additionally, this core will provide state-of-the art imaging services with developed expertise in advanced modalities specific for the analysis of the thyroid gland, such as anatomic imaging using high resolution, 3D mouse thyroid ultrasonography and functional imaging using integrated microCT/microSPECT/microPET. This latter imaging is capable of analyzing the same radiotracers in mice as are used clinically in human patients with thyroid cancer, including radioiodide to assess iodide uptake and retention, and fiuorodeoxyglucose to assess metabolic activity. Additional imaging modes available through the core include digital Faxitron high resolution radiography, high resolution small animal MRI, and whole animal or organ bioluminescence and fluorescence imaging. Other services that will be available to investigators include (1) technical expertise and support for the primary culture of mouse thyroid tumors; (2) support for studies on metastasis including orthotopic implantation of tumor tissue into the thyroid bed and injection of tumor cells into the tail vein and/or left cardiac ventricle to induce metastatic disease; and (3) support for the development of new transgenic and targeted alleles for novel mouse model development. Facilities will include a necropsy facility, automated immunostainers, microscopy facilities including a multi-headed microscope with video output for conferences, dedicated bioluminescent and fluorescent in vivo imaging instrumentation, dedicated high resolution/high frequency ultrasound equipment for small animals, digital gross and microscopic photography facilities, quantitative image analysis software, and mouse surgery and radiology facilities. Because the science in this project is spread among various models and physical locations, the availability of a single core to coordinate, analyze, and compare data will provide an essential resource and significantly enhance the quality and validity ofthe research data.