Stem Cell Core

核心A：虽然多能干细胞及其分化的后代在心脏移植方面有巨大的前景， 修复，他们的日常文化是苛刻的，需要相当多的专业知识和持续的关注， 质量控制根据我们以前对这些细胞的丰富经验，我们发现， 细胞培养工作的最佳方式是确保大规模生产的一致性和效率， 未分化和分化的细胞。因此，干细胞核心的主要目标是产生 大量未分化的胚胎干细胞（ESC），诱导多能干细胞（iPSC）， 间充质干细胞（MSC）和心脏衍生物分配给个人项目， 程序.核心小组还将与项目2的研究人员密切合作， 来自犬ESC的心肌细胞。最后，核心还将开发和共享优化的协议， ESCs和iPSCs的遗传修饰，包括通过以下途径产生细胞类型特异性报告细胞系： 在细菌染色体中的转基因（BAC）。总而言之，该核心将实现三个具体目标： 1)为个人项目提供完成其所需的干细胞和心脏衍生物， 2）开发BAC转基因并优化修饰的BAC向人类的递送， 胚胎干细胞和诱导多能干细胞），以及3）提供体外实验的培训和技术援助， 多能干细胞或其分化后代。

Core A: While pluripotent stem cells and their differentiated progeny have tremendous promise for cardiac repair, their routine culture is demanding and requires considerable expertise and continuous attention to quality control. Based on our extensive prior experience with these cells, we have found that centralization of cell culture efforts is the best way to ensure consistency and efficiency in the large-scale production of undifferentiated and differentiated cells. Hence, the primary goal ofthe Stem Cell Core will be to generate large numbers of undifferentiated embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs) and cardiac derivatives for distribution to the individual projects in this program. The Core will also work closely with investigators in Project 2 to optimize the derivation of cardiomyocytes from canine ESCs. Finally, the Core will also develop and share optimized protocols for the genetic modification of ESCs and iPSCs, including the generation of cell type specific reporter lines via transgenesis in bacterial artficial chromosomes (BACs). In sum, the Core will undertake three specific aims: 1) to provide the individual projects with the stem cells and cardiac derivatives needed to complete their respective aims, 2) to develop BAC transgenesis and optimize the delivery of modified BACs into human ESCs and iPSCs), and 3) to provide training and technical assistance with in vitro experiments involving pluripotent stem cells or their differentiated progeny.