IGF::OT::IGF DETECTION, ISOLATION, AND ANALYSIS OF SINGLE CTCS

该项目的目标是提供一个完全集成的clia认证技术，提供从人类标本中收获的单独鉴定的CTC的诊断特性。我们将使用一种新颖的专利芯片为基础的介电控制分离工艺（DEPArray）来鉴定和分离单个ctc，同时保持其功能性生物完整性。利用这项技术，我们开发了一种策略，将ctc分类为具有可识别特征的定义子集，这些特征对临床研究和诊断有用。本第一阶段合同的具体任务是：

The objective of this project is to deliver a fully integrated CLIA-certified technology that provides diagnostic characterization of individually identified CTC¿s harvested from human specimens. We will use a novel patented chip-based dielectrophoretic-controlled isolation process (DEPArray) to identify and isolate single CTCs while maintaining their functional biological integrity. Using this technology we have developed a strategy for classifying CTCs into defined subsets with identifiable characteristics useful for clinical research and diagnosis. The specific tasks for this Phase I contract are to: 1) Establish a robust method for comprehensive detection and isolation of single circulating tumor cells 2) Define and isolate specific subsets of CTCs based on extracellular antigen expression 3) Demonstrate the ability to perform molecular analysis on single cells isolated from subgroups of CTCs 4) Demonstrate reproducibility of an integrated ¿CTC Detection, Recovery, and Analysis¿ system This SBIR contract will enable development of a fully-integrated and robust cell-identification and separation system to support CTC cancer research and diagnostics. This novel technology promises to revolutionize the identification of new subsets of CTCs and also assign clinically useful characteristics, such as metastatic potential, to each subset of cells.