Tissue and Pathology Core

人类和小鼠胃肠道组织样本的获取、病理表征和分子分析是本SPORE应用程序所有项目的基础和组成部分。丹娜-法伯/哈佛癌症中心（DF/HCC）胃肠道（GI）癌症组织和病理核心的孢子将继续为孢子研究人员提供基础和高级病理服务，以支持每个研究项目中概述的研究，以及职业发展和发展项目。组织和病理中心将通过与胃肠道肿瘤库和DF/HCC病理标本定位器的互动，收集、储存和提供组织标本，进行组织学分析，并提供人类食管、结肠、胰腺和胃肠道间质肿瘤（gist）的解释性病理分析。核心将确保适当分类的胃肠道肿瘤组织样本被用于所有免疫组织化学和分子分析。此外，核心致力于胃肠道肿瘤的表征使用先进的分子病理学工具。将提供各种对成功进行小鼠和人类胃肠道肿瘤分子分析至关重要的服务，包括；实验研究中所有肿瘤样本的组织病理学检查和质量控制分析；构建组织微阵列以改进和简化所有免疫组织化学分析；冷冻和福尔马林固定石蜡包埋（FFPE）组织样本的选择和宏观切片，以确保在实验研究中使用适当的细胞区室；免疫组织化学（IHC）应用的抗体优化；对小鼠和人体组织中广泛的免疫组化染色进行性能和分析，以直接支持该项目；从所有组织类型（新鲜冷冻和存档FFPE）中提取核酸进行分离，用于分子分析。最后，核心将评估和实施新技术，如胃肠道肿瘤切片的体外培养及其随后的分子表征，以响应治疗，生物标志物的多路复用和复杂的细胞成像技术。

The acquisition, pathologic characterization and molecular analysis of human and mouse GI tissue samples is a fundamental and integral for all projects of this SPORE application. The Dana-Farber / Harvard Cancer Center (DF/HCC) SPORE in Gastrointestinal (GI) Cancer Tissue and Pathology Core will continue to provide basic and advance pathology services to SPORE investigators in support the studies outlined in each of the Research Projects, as well as in Career Development and Developmental Projects. The Tissue and Pathology Core will collect, store and provide tissue specimens, through interactions with GI Tumor banks and the DF/HCC Pathology Specimen Locator, perform histologic analyses and provide interpretive pathologic analysis of human esophageal, colonic, pancreatic and GI stromal tumors (GISTs). The Core will ensure that appropriately classified GI cancer tissue samples are used and supplied for all immunohistochemical and molecular analyses proposed. In addition, the Core is committed to the characterization of gastrointestinal neoplasms using advanced molecular pathology tools. A variety of services critical to successful molecular analysis of mouse and human GI tumors will be provided, including; histopathologic review and quality control analysis of all tumor samples utilized in experimental studies; construction of tissue micorarrays to improve and streamline all immunohistochemical analyses; selection and macrodlssection of frozen and formalin-fixed, paraffin-embedded (FFPE) tissue samples to ensure that appropriate cellular compartments are utilized in experimental studies; antibody optimizations for applications in immunohistochemistry (IHC); performance and analysis of a broad range of IHC stains in mouse and human tissues in direct support of the projects; and isolation of nuclei acid extractions from all tissue types (fresh frozen and archival FFPE) for molecular analyses. Finally, the Core will evaluate and implement novel technologies such as ex vivo incubation of GI tumor sections and their subsequent molecular characterization in response to treatment, multiplexing of biomarkers and sophisticated cellular imaging techniques.