Evaluation of Salmonella in Symptomatic and Asymptomatic Pets

项目摘要 本研究的目的是确定沙门氏菌在这两个地区的粪便样本中的流行率和类型。 有症状和无症状的动物被带到兽医诊所。沙门氏菌可以存在于许多 不同的环境，包括水、土壤、昆虫、粪便、野生和农场饲养的动物、生的食物、表面 和动物饲料。沙门氏菌病会导致人类和动物的胃肠道疾病。人类的症状 包括恶心、呕吐、腹部痉挛、腹泻、发烧和头痛。动物也可能表现出 胃肠道疾病，尽管它们可能携带沙门氏菌，但没有临床症状 疾病。自1999年以来，多起人类沙门氏菌病暴发与摄入 加拿大和美国被沙门氏菌污染的动物饲料。目前尚不清楚的是 受沙门氏菌污染的饲料对宠物健康的影响这种情况有多频繁？ 沙门氏菌病发生在宠物身上，它们多久一次会发展成携带者状态，它们在哪里庇护 细菌，但没有表现出疾病的临床症状？此外，沙门氏菌的各种血清型参与了 宠物疾病还没有得到充分的定性。为了进一步了解流行性出血热 饲料污染后感染沙门氏菌的动物，FDA兽医中心(CVM) 征求建议，以提供从以下地点获得的粪便样本中沙门氏菌流行率的信息 腹泻和非腹泻宠物寻求兽医护理..实验室将参与一项多中心研究 云服务器将协调参与者之间的方法。抽样方案和培养方法将是 在所有参与实验室中实现标准化。患者粪便样本将进行沙门氏菌培养 使用公认的和经过验证的方法。血清分型方法也将在 参与者。这些分离株将在研究完成后提交给云服务器。至少100 将在12个月内检查100例有症状和无症状的病例。参与 实验室将与其他合作协议实验室合作开发最终标准化的 他们的微生物分离方案。他们还将被要求参加一轮熟练程度考试 每年测试一次。此外，他们将被要求以商定的标准化格式每季度报告调查结果 由合作实验室提供。

Project Summary The objective of this study is to determine the prevalence and types of Salmonella in fecal samples from both symptomatic and asymptomatic animals brought to veterinary clinics. Salmonella can be present in many different environments, including water, soil, insects, feces, wild and farm raised animals, raw foods, surfaces and animal feeds. Salmonellosis causes gastrointestinal disease in humans and animals. Symptoms in people include nausea, vomiting, abdominal cramps, diarrhea, fever and headache. Animals may also show signs of gastrointestinal disease, although they may harbor Salmonella bacteria without showing signs of clinical disease. Since 1999, a number of human salmonellosis outbreaks have been correlated with ingestion of Salmonella-contaminated animal feeds in Canada and the United States. What remains generally unknown is the effect that Salmonella contaminated feeds has on the health of pets. How frequently do cases of Salmonellosis occur in pets, and how frequently do they develop a carrier state, where do they harbor the bacteria but fail to exhibit clinical signs of disease? In addition, the various serotypes of Salmonella involved in pet disease have not been adequately characterized. In order to gain further insight into the prevalence of Salmonella infected animals following feed contamination, the FDA Center for Veterinary Medicine (CVM) is seeking proposals that will provide information on the prevalence of Salmonella in fecal samples obtained from diarrheic and non-diarrheic pets seeking veterinary care.. Laboratories will participate in a multi-center study and CVM will coordinate methods among the participants. Sampling protocols and culture methods will be standardized throughout the participating laboratories. Patient fecal samples will be cultured for Salmonella from using well established and validated methods. Serotyping methods will also be standardized among the participants. These isolates will be submitted to CVM upon completion of the study. A minimum of 100 symptomatic and 100 asymptomatic cases will be examined during a 12 month period. Participating laboratories will collaborate with other cooperative agreement laboratories to develop a final standardized protocol for their microbiologic isolations. They will also be required to participate in one round of proficiency testing per year. Further they will be required to report findings quarterly in a standardized format agreed upon by the cooperative laboratories.