JHU Center for Neuroscience Research

C1a。多光子成像核心 C1A L？核心的建立和设置： 在这笔赠款和该机构提供的资金的支持下，多光子成像(MPI)核心JHU于2006年11月全面投入运行。在资助的第一年，完成了适合成像的专用空间的设计和翻新，购买和安装了仪器，并聘请和培训了一名全职技术员。Bergles博士担任该核心的主任，并由Dr。 Pucak说，他是该设施的核心经理。该中心的目标是提供对尖端仪器的访问，用于：1)以高分辨率分析蛋白质定位、蛋白质动力学和蛋白质-蛋白质相互作用；2)在活细胞和组织中执行多个荧光团的延时成像；以及3)结合荧光标记的蛋白质或离子指示剂染料的高分辨率成像 电生理监测电活动。MPI Core最初位于JHU医学院校区基础科学研究集群的Wood基础科学大楼的10层。在机构的支持下，这个设施于2009年9月沿着大厅搬迁到紧邻的临床前教学大楼10英寸层的新装修空间。这一举措扩大了 设施翻了一番，达到约500平方米这允许重组显微镜布局以更有效地使用(在安装第二个显微镜之后是必需的)、增加专用准备区域以及扩展图像处理套件。核心目前被分成一个大房间(约350平方米)。英国《金融时报》)里面有一张隔振台、两个显微镜和相关设备，以及一个外部前厅(约150平方米。英国《金融时报》)这是负责该设施日常运营的高级研究技术员的办公室，也是分析成像和电生理数据的场所。这个核心的原始仪器(蔡司LSM 510 NLO Meta)是由JHU SOM提供的资金提供的，核心的所有空间由神经科学部提供。该设施的日程安排由JHU神经科学门户网站提供的基于网络的交互式日历辅助。该中心与核心成员Bergles博士、Ginty博士、Huganir博士、Kolodkin(PI)博士和Sockanathan博士的实验室位于同一楼层，本提案中列出的其他调查人员如果在同一大楼或相邻大楼的其他楼层有实验室，则可以很容易地进入该中心。临床前教学大楼由两部电梯连接，并通过一座有盖的桥梁连接到JHU SOM的其他建筑，为临床和基础科学系的所有NINDS研究人员提供了方便地进入三个核心的通道。

C1A. MULTIPHOTON IMAGING CORE C1 A l ¿ Establishment and setup of the Core: The Multiphoton Imaging (MPI) Core JHU became fully operational in November 2006 with support from this grant and funds provided by the institution. During the first year of funding, design and renovation of dedicated space suitable for imaging was completed, instrumentation was purchased and installed, and a fulltime technician was hired and trained. Dr. Bergles serves as the Director of this Core and is assisted by Dr. Pucak, who serves as Core Manager of the facility. The goals of this Core are to provide access to cuttingedge instrumentation for: 1) analyzing protein localization, protein dynamics, and protein-protein interactions with high resolution; 2) performing time-lapse imaging of multiple fluorophores in living cells and tissues; and 3) combining high-resolution imaging of fluorescently tagged proteins or ion indicator dyes with electrophysiological monitoring of electrical activity. The MPI Core was originally located on the 10th floor of the Wood Basic Science building in the Basic Science research cluster on the JHU medical campus. With institutional support, this facility was relocated in September 2009 down the hall to newly renovated space on the 10"^ floor of the immediately adjacent Preclinical Teaching Building. This move increased the size of the facility 2-fold to ~500 sq. ft., which allowed reorganization of the microscope layout for more efficient use (necessitated after the installation of the second microscope), addition of a dedicated preparation area, and expansion of the image processing suite. The Core is currently divided into one large room (~350 sq. ft.) that houses a vibration isolation table, two microscopes and associated equipment, and an outer vestibule (~150 sq. ft.) that is used as an office by the senior research technician responsible for day-to-day operation of the facility and as a site for analysis of imaging and electrophysiology data. The original instrumentation for this Core (Zeiss LSM 510 NLO Meta) was provided by funds from the JHU SOM, and all space for the Core was provided by the Department of Neuroscience. Scheduling for this facility is aided by an interactive web-based calendar accessible through the JHU Neuroscience portal. This Core is located on the same floor as the laboratories of Core members Drs. Bergles, Ginty, Huganir, Kolodkin (PI) and Sockanathan, and it is readily accessible by the other investigators listed in this proposal who have laboratories on other floors in the same building or in adjacent buildings. The Preclinical Teaching Building is sen/ed by two elevators and is connected by a covered bridge to other buildings at JHU SOM, providing convenient access to the three Cores for all NINDS investigators in clinical and basic science departments.